Method
Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera)
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* Corresponding author: Denis Tagu denis.tagu@rennes.inra.fr
1 INRA Rennes, UMR INRA-Agrocampus BiO3P, BP 35327, F-35653 Le Rheu Cedex, France
2 INRA, URGI - Genoplante Info, Infobiogen, 523 place des Terrasses, F-91000 Evry, France
3 Biochemistry Department, University of Otago, PO Box 56, Dunedin, New Zealand
4 GENOSCOPE and CNRS UMR 8030, Centre National de Séquençage, 2 rue Gaston Crémieux, F-91000 Evry Cedex, France
5 USDA, Agricultural Research Service, US Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA
6 Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
7 Institut Cavanilles de Biodiversitat i Biologia Evolutiva (ICBIBE), Universitat de Valencia, Apartado de Correos 2085, 46071 Valencia, Spain
8 Environmental Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 Japan
9 INRA Lyon, UMR INRA-INSA BF2I, INSA Bâtiment Louis-Pasteur, 20 avenue A. Einstein, 69621 Villeurbanne cedex, France
10 Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA
11 Current address: Instituto Valenciano de Investigaciones Agrarias (IVIA), Proteccion Vegetal y Biotecnologia, Lab Entomologia, 46113 Moncada, Valencia, Spain
Genome Biology 2006, 7:R21 doi:10.1186/gb-2006-7-3-r21
Published: 10 March 2006Abstract
Aphids are the leading pests in agricultural crops. A large-scale sequencing of 40,904 ESTs from the pea aphid Acyrthosiphon pisum was carried out to define a catalog of 12,082 unique transcripts. A strong AT bias was found, indicating a compositional shift between Drosophila melanogaster and A. pisum. An in silico profiling analysis characterized 135 transcripts specific to pea-aphid tissues (relating to bacteriocytes and parthenogenetic embryos). This project is the first to address the genetics of the Hemiptera and of a hemimetabolous insect.