Open Access Method

Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera)

Beatriz Sabater-Muñoz111, Fabrice Legeai2, Claude Rispe1, Joël Bonhomme1, Peter Dearden3, Carole Dossat4, Aymeric Duclert2, Jean-Pierre Gauthier1, Danièle Giblot Ducray1, Wayne Hunter5, Phat Dang5, Srini Kambhampati6, David Martinez-Torres7, Teresa Cortes7, Andrès Moya7, Atsushi Nakabachi8, Cathy Philippe2, Nathalie Prunier-Leterme1, Yvan Rahbé9, Jean-Christophe Simon1, David L Stern10, Patrick Wincker4 and Denis Tagu1*

Author Affiliations

1 INRA Rennes, UMR INRA-Agrocampus BiO3P, BP 35327, F-35653 Le Rheu Cedex, France

2 INRA, URGI - Genoplante Info, Infobiogen, 523 place des Terrasses, F-91000 Evry, France

3 Biochemistry Department, University of Otago, PO Box 56, Dunedin, New Zealand

4 GENOSCOPE and CNRS UMR 8030, Centre National de Séquençage, 2 rue Gaston Crémieux, F-91000 Evry Cedex, France

5 USDA, Agricultural Research Service, US Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945, USA

6 Department of Entomology, Kansas State University, Manhattan, KS 66506, USA

7 Institut Cavanilles de Biodiversitat i Biologia Evolutiva (ICBIBE), Universitat de Valencia, Apartado de Correos 2085, 46071 Valencia, Spain

8 Environmental Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 Japan

9 INRA Lyon, UMR INRA-INSA BF2I, INSA Bâtiment Louis-Pasteur, 20 avenue A. Einstein, 69621 Villeurbanne cedex, France

10 Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA

11 Current address: Instituto Valenciano de Investigaciones Agrarias (IVIA), Proteccion Vegetal y Biotecnologia, Lab Entomologia, 46113 Moncada, Valencia, Spain

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Genome Biology 2006, 7:R21  doi:10.1186/gb-2006-7-3-r21

Published: 10 March 2006

Additional files

Additional data File 1:

A list of the 25 most abundant A. pisum unique transcripts from the 12,082 collection.

Format: XLS Size: 21KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional data File 2:

A list of the 921 perfect microsatellite motifs found in the 12,082 A. pisum contigs

Format: XLS Size: 130KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional data File 3:

A table giving the description by cDNA libraries of the 135 contigs specific to a given A. pisum tissue

Format: XLS Size: 175KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data