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Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins

Patrick Meraldi12, Andrew D McAinsh13, Esther Rheinbay1 and Peter K Sorger1*

  • * Corresponding author: Peter K Sorger psorger@mit.edu

  • † Equal contributors

Author Affiliations

1 Department of Biology, Massachusetts Institute of Technology, Massachusetts Ave., Cambridge, MA 02139, USA

2 Institute of Biochemistry, ETH Zurich, Schafmattstr.,18 CH-8093 Zurich, Switzerland

3 Chromosome Segregation Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK

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Genome Biology 2006, 7:R23  doi:10.1186/gb-2006-7-3-r23

Published: 22 March 2006

Abstract

Background

Kinetochores are large multi-protein structures that assemble on centromeric DNA (CEN DNA) and mediate the binding of chromosomes to microtubules. Comprising 125 base-pairs of CEN DNA and 70 or more protein components, Saccharomyces cerevisiae kinetochores are among the best understood. In contrast, most fungal, plant and animal cells assemble kinetochores on CENs that are longer and more complex, raising the question of whether kinetochore architecture has been conserved through evolution, despite considerable divergence in CEN sequence.

Results

Using computational approaches, ranging from sequence similarity searches to hidden Markov model-based modeling, we show that organisms with CENs resembling those in S. cerevisiae (point CENs) are very closely related and that all contain a set of 11 kinetochore proteins not found in organisms with complex CENs. Conversely, organisms with complex CENs (regional CENs) contain proteins seemingly absent from point-CEN organisms. However, at least three quarters of known kinetochore proteins are present in all fungi regardless of CEN organization. At least six of these proteins have previously unidentified human orthologs. When fungi and metazoa are compared, almost all have kinetochores constructed around Spc105 and three conserved multi-protein linker complexes (MIND, COMA, and the NDC80 complex).

Conclusion

Our data suggest that critical structural features of kinetochores have been well conserved from yeast to man. Surprisingly, phylogenetic analysis reveals that human kinetochore proteins are as similar in sequence to their yeast counterparts as to presumptive Drosophila melanogaster or Caenorhabditis elegans orthologs. This finding is consistent with evidence that kinetochore proteins have evolved very rapidly relative to components of other complex cellular structures.