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Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins

Patrick Meraldi1,2†, Andrew D McAinsh1,3†, Esther Rheinbay1 and Peter K Sorger1*

  • * Corresponding author: Peter K Sorger psorger@mit.edu

  • † Equal contributors

1 Department of Biology, Massachusetts Institute of Technology, Massachusetts Ave., Cambridge, MA 02139, USA

2 Institute of Biochemistry, ETH Zurich, Schafmattstr.,18 CH-8093 Zurich, Switzerland

3 Chromosome Segregation Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK

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Genome Biology 2006, 7:R23 doi:10.1186/gb-2006-7-3-r23

Published: 22 March 2006

Additional files

Additional File 1:

Accession numbers of all proteins that are used in this study.

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Open Data

Additional File 2:

Identification of a potential ortholog of the DASH complex subunit Spc34 in humans. S. cerevisiae Spc34 was aligned with five fungal and four metazoan sequences. Percentages denote the degree of similarity of successive sequence blocks (black boxes). White letters on black denote identical residues, white letters on green, identical residues in ≤ 80% of the organisms and black letters on green, similar residues in ≤ 80% of the organisms. Accession numbers are described in additional data file 1.

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Additional File 3:

Identification of E. cuniculi kinetochore proteins. Multiple sequence alignments of the Ndc80, Nuf2, Nnf1, Mis12Mtw1, CENP-CMif2 and Spc105 proteins amongst five fungi and E. cuniculi. Percentages denote the degree of similarity of successive sequence blocks (black boxes). White letters on black denote identical residues, white letters on green, identical residues in ≥ 80% of the organisms and black letters on green, similar residues in ≥ 80% of the organisms. Accession numbers are described in additional data file 1.

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Additional File 4:

Amino acid similarities used in all multiple sequence alignments.

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Additional File 5:

Homology blocks used in phylogenetic analysis.

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