Identification of a minimal kinetochore in E. cuniculi. (a) HMM described in Figure 1a, b failed to find a CDEI-II-III structure in the genome of E. cuniculi. The Green bar indicates point CENs identified and black bars the number of chromosomes. (b) Speculative model of E. cuniculi kinetochore subunit organization. Proteins colored in pink, blue, green or yellow represent components of the DNA binding, linker, regulatory or microtubule-binding layers, respectively, based on kinetochore organization in S. cerevisiae. Potential multi-protein complexes are highlighted with a grey box. (c) Sequence alignment of fungal, metazoan and plantae Cdc20 showing the conserved Mad2 binding site. Note: E. cuniculi lacks both the conserved Mad2 binding site and an ortholog of the Mad2 protein. Schematic drawings indicate the length of the S. cerevisiae and E. cuniculi proteins, the position of the WD-domain (black box) and the position where Mad2 binds.
Meraldi et al. Genome Biology 2006 7:R23 doi:10.1186/gb-2006-7-3-r23