Figure 2.

Purification of WW domain fusion proteins. Coomassie-stained SDS-PAGE gel of WW domain fusion proteins following protein purification (top panels), western blot detection of fusion protein expression with anti-GST antibody (left middle panel) or anti-myc antibody (right middle panel), and biotinylation of fusion proteins observed by binding of HRP-conjugated streptavidin (bottom panels) are shown.