Figure 3.

Protein microarray data and the Rsp5 network. (a) A microarray was probed with the first WW domain from Rsp5 and interactions were visualized via application of dye-labeled streptavidin and fluorescent scanning. Following data processing, two proteins (Ubc6 and Oye3) had signals above background. Control proteins (dye-labeled and biotinylated proteins) are indicated. (b) Interactions involving the WW domains from Rsp5. A total of 124 proteins were identified using the WW domains from Rsp5. Functional annotations are superimposed on the network using filled circles and outlines.