Old friends: confirmation of microarray results. Real-time RT-PCR was performed for (a) FOXP3, (b) CTLA4, (c) CCR7, and RPS9 (data not shown) expression in MACS separated human CD4+CD25+ TReg and CD4+CD25- naïve T cells. Following normalization to RPS9, relative mRNA amounts in CD4+CD25+ TReg cells were adjusted to corresponding expression levels in CD4+CD25- naïve T cells and expressed as fold changes. Real-time RT-PCR results, indicated by black bars, were compared with fold changes arising from the Human TReg Chip (represented by grey bars). The healthy donors, randomly chosen, are specified by letters (see Table 1). RT-PCR, reverse transcription polymerase chain reaction.
Pfoertner et al. Genome Biology 2006 7:R54 doi:10.1186/gb-2006-7-7-r54