Figure 5.

New players: confirmation of microarray results. Real-time RT-PCR was performed for (a) TNFRSF1B, (b) TRAF1, and (c) LGALS3 expression in MACS isolated human CD4⁺CD25⁺ T_Reg and CD4⁺CD25^- naïve T cells. Fold changes were calculated as described for Figure 4. Real-time RT-PCR results (black bars) were compared with fold changes arising from the Human T_Reg Chip (white bars). The healthy donors are specified by letters (see Table 1). RT-PCR, reverse transcription polymerase chain reaction.