A method for high-throughput gene expression signature analysis

David Peck¹ , Emily D Crawford¹ , Kenneth N Ross¹ , Kimberly Stegmaier² , Todd R Golub¹^,2^,3 and Justin Lamb¹

¹Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA

²Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA

³Howard Hughes Medical Institute, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA

author email corresponding author email

Genome Biology 2006, 7:R61doi:10.1186/gb-2006-7-7-r61


Published:	19 July 2006

Subject areas: Methods, Genome studies

Abstract

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.