Method
A method for high-throughput gene expression signature analysis
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* Corresponding author: Justin Lamb justin@broad.mit.edu
1 Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA
2 Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA
3 Howard Hughes Medical Institute, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA
Genome Biology 2006, 7:R61 doi:10.1186/gb-2006-7-7-r61
Published: 19 July 2006Abstract
Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.