Table 1 |
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|
Combinatorial RNAi effectively generates additive phenotypes |
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|
Gene1 |
Gene2 |
Wild-type |
rrf-3 |
||
|
|
|||||
|
Pheno Gene1 |
Pheno Gene2 |
Pheno Gene1 |
Pheno Gene2 |
||
|
|
|||||
|
lin-31 |
- |
5% |
- |
35% |
- |
|
sma-4 |
- |
100% |
- |
100% |
- |
|
unc-22 |
- |
100% |
- |
100% |
- |
|
lon-2 |
- |
100% |
- |
100% |
- |
|
lin-31 |
sma-4 |
2% |
100% |
20% |
100% |
|
lin-31 |
unc-22 |
2% |
100% |
26% |
100% |
|
lin-31 |
lon-2 |
4% |
100% |
13% |
100% |
|
sma-4 |
unc-22 |
100% |
100% |
100% |
100% |
|
sma-4 |
lon-2 |
100% |
0% |
100% |
0% |
|
unc-22 |
lon-2 |
100% |
100% |
100% |
100% |
|
|
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|
Wild-type and RNA interference (RNAi)-hypersensitive rrf-3 worms, respectively, were fed on selected bacterial strains of the C. elegans RNAi feeding library [2] targeting the genes lin-31, sma-4, unc-22, and lon-2. Independent RNAi phenotypes (Pheno Gene1, Pheno Gene2) were assessed when each gene was targeted individually and also for all possible pair-wise combinations of genes. Percentages represent penetrance of phenotypes. |
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|
Tischler et al. Genome Biology 2006 7:R69 doi:10.1186/gb-2006-7-8-r69 |
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