Table 1

Combinatorial RNAi effectively generates additive phenotypes

Gene1

Gene2

Wild-type

rrf-3


Pheno Gene1

Pheno Gene2

Pheno Gene1

Pheno Gene2


lin-31

-

5%

-

35%

-

sma-4

-

100%

-

100%

-

unc-22

-

100%

-

100%

-

lon-2

-

100%

-

100%

-

lin-31

sma-4

2%

100%

20%

100%

lin-31

unc-22

2%

100%

26%

100%

lin-31

lon-2

4%

100%

13%

100%

sma-4

unc-22

100%

100%

100%

100%

sma-4

lon-2

100%

0%

100%

0%

unc-22

lon-2

100%

100%

100%

100%


Wild-type and RNA interference (RNAi)-hypersensitive rrf-3 worms, respectively, were fed on selected bacterial strains of the C. elegans RNAi feeding library [2] targeting the genes lin-31, sma-4, unc-22, and lon-2. Independent RNAi phenotypes (Pheno Gene1, Pheno Gene2) were assessed when each gene was targeted individually and also for all possible pair-wise combinations of genes. Percentages represent penetrance of phenotypes.

Tischler et al. Genome Biology 2006 7:R69   doi:10.1186/gb-2006-7-8-r69

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