Table 2

Combinatorial RNAi can identify known synthetic lethal interactions
Strain
 Interaction Gene1 + Gene 2
 Gene1
 Gene2
 Gene1 + 2
 Syn
 p value





BS
 ES
 BS
 ES
 BS
 ES
BS
 ES
Wild-type
 mec-8 + sym-1
 88
 99
 82
 98
 78
 92
 Yes
 5.5 × 10-01
 1.3 × 10-02

sop-3 + sop-1
 91
 100
 94
 99
 79
 90
 Yes
 2.8 × 10-01
 8.5 × 10-04

tbx-8 + tbx-9
 83
 99
 78
 97
 52
 11
 Yes
 7.3 × 10-02
 1.4 × 10-24

hlh-1 + unc-120
 91
 99
 76
 99
 28
 91
 Yes
 5.2 × 10-05
 1.2 × 10-02

hlh-1 + hnd-1
 88
 97
 75
 98
 62
 81
 Yes
 6.6 × 10-01
 5.7 × 10-03

unc-120 + hnd-1
 54
 100
 74
 98
 36
 100
 No
 6.4 × 10-01
 1.9 × 10-01

egl-27 + egr-1
 93
 99
 79
 90
 90
 89
 No
 6.0 × 10-02
 7.4 × 10-01
rrf-3
 mec-8 + sym-1
 67
 73
 61
 73
 59
 16
 Yes
 3.3 × 10-01
 3.0 × 10-06

sop-3 + sop-1
 82
 100
 85
 96
 41
 75
 Yes
 3.1 × 10-04
 5.7 × 10-06

tbx-8 + tbx-9
 96
 99
 86
 92
 59
 2
 Yes
 8.6 × 10-03
 6.3 × 10-27

hlh-1 + unc-120
 90
 90
 31
 99
 1
 64
 Yes
 8.1 × 10-06
 2.9 × 10-03

hlh-1 + hnd-1
 86
 87
 82
 94
 42
 24
 Yes
 1.6 × 10-03
 8.2 × 10-14

unc-120 + hnd-1
 33
 100
 87
 94
 7
 98
 Yes
 5.7 × 10-04
 4.8 × 10-02

egl-27 + egr-1
 97
 99
 83
 93
 73
 62
 Yes
 2.9 × 10-01
 5.7 × 10-08

Quantitative analysis of known synthetic lethal interactions (Interaction Gene1 + Gene2; see below for references) after combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. Percentages of average wild-type brood size (BS) and embryonic survival (ES) rates resulting from RNAi targeting each gene individually (Gene1 or Gene2) as well as targeting both genes simultaneously (Gene1 + 2) are shown. A synthetic interaction (Syn) was scored positive for p < 5.0 × 10-02 (by Student's t-test). References for genes tested: mec-8 + sym-1 [21]; sop-3 + sop-1 [22]; tbx-8 + tbx-9 [23]; hlh-1 + unc-120, hlh-1 + hnd-1, unc-120 + hnd-1 [24]; and egl-27 + egr-1 [25].

Tischler et al. Genome Biology 2006 7:R69   doi:10.1186/gb-2006-7-8-r69