Table 2

Combinatorial RNAi can identify known synthetic lethal interactions

Strain

Interaction Gene1 + Gene 2

Gene1

Gene2

Gene1 + 2

Syn

p value



BS

ES

BS

ES

BS

ES

BS

ES


Wild-type

mec-8 + sym-1

88

99

82

98

78

92

Yes

5.5 × 10-01

1.3 × 10-02

sop-3 + sop-1

91

100

94

99

79

90

Yes

2.8 × 10-01

8.5 × 10-04

tbx-8 + tbx-9

83

99

78

97

52

11

Yes

7.3 × 10-02

1.4 × 10-24

hlh-1 + unc-120

91

99

76

99

28

91

Yes

5.2 × 10-05

1.2 × 10-02

hlh-1 + hnd-1

88

97

75

98

62

81

Yes

6.6 × 10-01

5.7 × 10-03

unc-120 + hnd-1

54

100

74

98

36

100

No

6.4 × 10-01

1.9 × 10-01

egl-27 + egr-1

93

99

79

90

90

89

No

6.0 × 10-02

7.4 × 10-01

rrf-3

mec-8 + sym-1

67

73

61

73

59

16

Yes

3.3 × 10-01

3.0 × 10-06

sop-3 + sop-1

82

100

85

96

41

75

Yes

3.1 × 10-04

5.7 × 10-06

tbx-8 + tbx-9

96

99

86

92

59

2

Yes

8.6 × 10-03

6.3 × 10-27

hlh-1 + unc-120

90

90

31

99

1

64

Yes

8.1 × 10-06

2.9 × 10-03

hlh-1 + hnd-1

86

87

82

94

42

24

Yes

1.6 × 10-03

8.2 × 10-14

unc-120 + hnd-1

33

100

87

94

7

98

Yes

5.7 × 10-04

4.8 × 10-02

egl-27 + egr-1

97

99

83

93

73

62

Yes

2.9 × 10-01

5.7 × 10-08


Quantitative analysis of known synthetic lethal interactions (Interaction Gene1 + Gene2; see below for references) after combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. Percentages of average wild-type brood size (BS) and embryonic survival (ES) rates resulting from RNAi targeting each gene individually (Gene1 or Gene2) as well as targeting both genes simultaneously (Gene1 + 2) are shown. A synthetic interaction (Syn) was scored positive for p < 5.0 × 10-02 (by Student's t-test). References for genes tested: mec-8 + sym-1 [21]; sop-3 + sop-1 [22]; tbx-8 + tbx-9 [23]; hlh-1 + unc-120, hlh-1 + hnd-1, unc-120 + hnd-1 [24]; and egl-27 + egr-1 [25].

Tischler et al. Genome Biology 2006 7:R69   doi:10.1186/gb-2006-7-8-r69

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