Table 3 |
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|
Genetic interactions of synthetic multivulval genes can be recapitulated by combinatorial RNAi |
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|
SynMuv gene |
Predicted gene |
Locus |
SynMuv pathway |
Wild-type |
rrf-3 |
|
|
|||||
|
lin-15B |
T27C4.4 |
egr-1 |
A |
- |
- |
|
ZK678.1 |
lin-15A |
A |
Muv |
Muv |
|
|
K12C11.2 |
smo-1 |
A, B |
ns |
ns |
|
|
W02A11.4 |
uba-2 |
A, B |
Muv |
Muv |
|
|
lin-15A |
K12C11.2 |
smo-1 |
A, B |
ns |
ns |
|
W02A11.4 |
uba-2 |
A, B |
- |
Muv |
|
|
C32F10.2 |
lin-35 |
B |
Muv |
Muv |
|
|
C47D12.1 |
trr-1 |
B |
ns |
ns |
|
|
C53A5.3 |
hda-1/gon-10 |
B |
ns |
ns |
|
|
E01A2.4 |
B |
- |
- |
||
|
F44B9.6 |
lin-36 |
B |
- |
Muv |
|
|
JC8.6 |
B |
ns |
ns |
||
|
K07A1.12 |
lin-53/rba-2 |
B |
ns |
ns |
|
|
M04B2.1 |
mep-1/gei-2 |
B |
- |
Muv |
|
|
R05D3.11 |
met-2 |
B |
- |
Muv |
|
|
R06C7.7 |
rls-1/lin-61 |
B |
Muv |
Muv |
|
|
W01G7.3 |
B |
ns |
ns |
||
|
W07B3.2 |
gei-4 |
B |
ns |
ns |
|
|
Y71G12B.9 |
B |
- |
Muv |
||
|
Y1O2A5C.18 |
efl-1 |
B |
Muv |
Muv |
|
|
ZK632.13 |
lin-52 |
B |
Muv |
Muv |
|
|
ZK637.7 |
lin-9 |
B |
Muv |
Muv |
|
|
ZK662.4 |
lin-15B |
B |
Muv |
Muv |
|
|
|
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|
Previously studied synthetic multivulval (synMuv) genes were targeted by combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. We show predicted gene name, its corresponding genetic locus name, a definition of the gene as a component of either the synMuv A (A), synMuv B (B), or both (A, B) pathways. All synMuv A genes were targeted by RNAi in combination with a double-stranded (ds)RNA-expressing strain targeting the synMuv B gene lin-15B; corresponding experiments were performed with synMuv B genes and a dsRNA-expressing strain targeting lin-15A. In both cases, worms were scored for the presence of the multivulva (Muv) phenotype. -, absence of Muv phenotype; ns, not scored (RNAi resulted in embryonic lethality or sterility). |
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|
Tischler et al. Genome Biology 2006 7:R69 doi:10.1186/gb-2006-7-8-r69 |
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