Table 3

Genetic interactions of synthetic multivulval genes can be recapitulated by combinatorial RNAi

SynMuv gene

Predicted gene

Locus

SynMuv pathway

Wild-type

rrf-3


lin-15B

T27C4.4

egr-1

A

-

-

ZK678.1

lin-15A

A

Muv

Muv

K12C11.2

smo-1

A, B

ns

ns

W02A11.4

uba-2

A, B

Muv

Muv

lin-15A

K12C11.2

smo-1

A, B

ns

ns

W02A11.4

uba-2

A, B

-

Muv

C32F10.2

lin-35

B

Muv

Muv

C47D12.1

trr-1

B

ns

ns

C53A5.3

hda-1/gon-10

B

ns

ns

E01A2.4

B

-

-

F44B9.6

lin-36

B

-

Muv

JC8.6

B

ns

ns

K07A1.12

lin-53/rba-2

B

ns

ns

M04B2.1

mep-1/gei-2

B

-

Muv

R05D3.11

met-2

B

-

Muv

R06C7.7

rls-1/lin-61

B

Muv

Muv

W01G7.3

B

ns

ns

W07B3.2

gei-4

B

ns

ns

Y71G12B.9

B

-

Muv

Y1O2A5C.18

efl-1

B

Muv

Muv

ZK632.13

lin-52

B

Muv

Muv

ZK637.7

lin-9

B

Muv

Muv

ZK662.4

lin-15B

B

Muv

Muv


Previously studied synthetic multivulval (synMuv) genes were targeted by combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. We show predicted gene name, its corresponding genetic locus name, a definition of the gene as a component of either the synMuv A (A), synMuv B (B), or both (A, B) pathways. All synMuv A genes were targeted by RNAi in combination with a double-stranded (ds)RNA-expressing strain targeting the synMuv B gene lin-15B; corresponding experiments were performed with synMuv B genes and a dsRNA-expressing strain targeting lin-15A. In both cases, worms were scored for the presence of the multivulva (Muv) phenotype. -, absence of Muv phenotype; ns, not scored (RNAi resulted in embryonic lethality or sterility).

Tischler et al. Genome Biology 2006 7:R69   doi:10.1186/gb-2006-7-8-r69

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