Research
Sex-specific expression of alternative transcripts in Drosophila
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* Corresponding author: Lauren M McIntyre mcintyre@ufl.edu
1 Department of Molecular Genetics and Microbiology, 1376 Mowry Road room 116, University of Florida, Gainesville, FL 32611, USA
2 Computational Genomics, 901 West State Street, Purdue University, West Lafayette, IN 47907, USA
3 Section of Evolution and Ecology, One Shields Avenue, University of California, Davis, California 95616, USA
4 Department of Horticulture, 625 Agriculture Mall Dr., Purdue University, West Lafayette, IN 47907, USA
5 Department of Agronomy, 915 West State Street, Purdue University, West Lafayette, IN 47907, USA
6 Department of Zoology, 223 Bartram Hall, University of Florida, Gainesville, FL 32611, USA
7 School of Biological Sciences, 335 Mant, University of Nebraska, Lincoln, NE 68588, USA
8 Center for Genetics and Development, One Shields Avenue, University of California, Davis, California, 95616, USA
Genome Biology 2006, 7:R79 doi:10.1186/gb-2006-7-8-r79
Published: 25 August 2006Additional files
Additional data file 1:
Microarray results for several sets of genes for which we had a priori expectations of sex-biased expression
Format: XLS Size: 74KB Download file
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Additional data file 2:
Actual_set_id is the unique identifier for each probe that hybridizes to the same set of transcripts, and actuals_cluster_id is a unique identifier that groups probes based upon CG number. Probeuid is the unique identifier for that probe sequence.
Format: CSV Size: 9MB Download file
Additional data file 3:
The P values obtained from the ANOVA are given with the notation p<effect>. The CG number is given in actuals and the model used for analysis (Single transcript/multiple transcript) is given in the final column.
Format: CSV Size: 3.1MB Download file
Additional data file 4:
Results of analysis based upon the probe level, as well as annotations from FlyBase
Format: CSV Size: 5.3MB Download file
Additional data file 5:
The columns are, in order: probe ID; gene name; whether hybridization signal detected by that probe is greater in males or females; log-transformed female/male expression ratio for each probe; probe set ID; class of probe (global or local); P value for the sex by probe set interaction; and the list of transcripts targeted by each probe. See text for further details.
Format: XLS Size: 141KB Download file
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Additional data file 6:
We give the probe sequences used, all qPCR results as well as the original array results to facilitate comparison. The P values of the Likelihood Ratio Test for a significant probe-sex interaction are also given. Note that for genes where only one transcript was tested, the test of the interaction between transcript and sex is not applicable (NA).
Format: XLS Size: 38KB Download file
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