|
Experimental conditions and statistics on database searches of four individual experiments using a single urine sample |
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| In-gel 1 |
In-gel 2 |
In-solution 1 |
In-solution 2 |
|
|
|
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| Urinary protein |
150 μg |
150 μg |
125 μg |
125 μg |
| Albumin removal |
- |
- |
+ |
+ |
| Protein separation |
Invitrogen NuPAGE 4-12% Bis-Tris 1D gel |
Agilent mRP-C18 column |
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| Number of fraction |
14 |
10 |
22 |
22 |
| Digestion |
In-gel |
In-gel |
In-solution |
In-solution |
| Denaturant |
50% Trifluoroethanol |
6 mol/l Urea + 2 mol/l thiourea |
||
| LC gradient time |
100 min |
140 min |
100 min |
100 min |
| Identified IT-MS2 spectra by Mascota |
16,219 |
10,535 |
13,367 |
10,175 |
| Number of unique peptidesa |
4504 |
3853 |
3164 |
2637 |
| Number of identified proteinsa |
759 |
815 |
656 |
580 |
| Total number of unique peptidesa |
8041 |
|||
| Total number of identified proteinsa |
1281 |
|||
|
aApplied criteria are described in the Materials and methods section. 1D, one-dimensional; LC, liquid chromatography; MS, mass spectrometry. | ||||
Adachi et al. Genome Biology 2006 7:R80 doi:10.1186/gb-2006-7-9-r80 |
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