Table 2

Experimental conditions and statistics on database searches of 10 slices of pooled urine sample

Pooled 1

Pooled 2

Pooled 3

Pooled 4

Pooled 5

Pooled 6

Pooled 7

Pooled 8

Pooled 9

Pooled 10


Protein separation

Invitrogen NuPAGE 4-12% Bis-Tris 1D Gel

Digestion

In-gel

LC gradient time

140 min

Identified IT-MS2 spectra by Mascot

42,578

36,288

46,328

42,664

48,938

46,529

48,101

50,654

26,607

26,817

Number of unique peptidesa

777

1133

1841

1114

1591

2493

2179

878

1671

2006

Number of identified proteinsa

125

186

290

186

229

302

239

96

206

153

Total number of unique peptidesa

9737

Total number of identified proteinsa

1055


aApplied criteria are described in the Materials and methods section.

Adachi et al. Genome Biology 2006 7:R80   doi:10.1186/gb-2006-7-9-r80

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