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Table 2
Experimental conditions and statistics on database searches of 10 slices of pooled urine sample
	Pooled 1	Pooled 2	Pooled 3	Pooled 4	Pooled 5	Pooled 6	Pooled 7	Pooled 8	Pooled 9	Pooled 10

Protein separation	Invitrogen NuPAGE 4-12% Bis-Tris 1D Gel
Digestion	In-gel
LC gradient time	140 min
Identified IT-MS²spectra by Mascot	42,578	36,288	46,328	42,664	48,938	46,529	48,101	50,654	26,607	26,817
Number of unique peptides^a	777	1133	1841	1114	1591	2493	2179	878	1671	2006
Number of identified proteins^a	125	186	290	186	229	302	239	96	206	153
Total number of unique peptides^a	9737
Total number of identified proteins^a	1055

^aApplied criteria are described in the Materials and methods section.
Adachi et al. Genome Biology 2006 7:R80 doi:10.1186/gb-2006-7-9-r80

Table 2