RT-PCR on floral organ abscission zones representing five unique stages of development. Expression of 43 PGs is examined in the abscission zones at five different stages of floral organ abscission as determined by position on the inflorescence, where position one represents anthesis and larger numbers are progressively older flowers (a). Five developmental stages were examined with the RT-PCR; i (position1/2) and ii (position 4/5), pre-abscission, iii (position 7/8), during abscission, iv (position 0/11), and v (position 13/14) post-abscission. Expression during the abscission process is classified into nine different unique patterns shown in (b) to (j); the gene names are provided in 7. PGs specifically up-regulated during the abscission process are shown with RT-PCR products (k).
Kim et al. Genome Biology 2006 7:R87 doi:10.1186/gb-2006-7-9-r87