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Open AccessMethod

An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

John Wang* 1 email, Stephanie Jemielity* 1 email, Paolo Uva2 email, Yannick Wurm1 email, Johannes Gräff3 email and Laurent Keller1 email

1Department of Ecology and Evolution, University of Lausanne, 1015 Lausanne, Switzerland

2Istituto di Ricerche di Biologia Molecolare, Merck Research Laboratories, 00040 Pomezia, Rome, Italy

3Brain Research Institute, University of Zürich/Swiss Federal Institute of Technology, 8057 Zürich, Switzerland

author email corresponding author email* Contributed equally

Genome Biology 2007, 8:R9doi:10.1186/gb-2007-8-1-r9

Published: 15 January 2007

Subject areas: Genome studies, Molecular biology

Abstract

Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.


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