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An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

John Wang1*, Stephanie Jemielity1, Paolo Uva2, Yannick Wurm1, Johannes Gräff3 and Laurent Keller1

Author affiliations

1 Department of Ecology and Evolution, University of Lausanne, 1015 Lausanne, Switzerland

2 Istituto di Ricerche di Biologia Molecolare, Merck Research Laboratories, 00040 Pomezia, Rome, Italy

3 Brain Research Institute, University of Zürich/Swiss Federal Institute of Technology, 8057 Zürich, Switzerland

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Citation and License

Genome Biology 2007, 8:R9  doi:10.1186/gb-2007-8-1-r9

Published: 15 January 2007

Abstract

Ants display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.