Table 1 |
|
|
Fire ant EST and assembly statistics |
|
| Total number of sequence reads |
28,133 |
| cDNA clones sequenced from 5' end |
22,560 |
| Extra reads due to re-sequencing |
5,573 |
| High-quality sequences after filtering* |
21,715 |
| Average EST size after trimming (bp) |
522.4 |
| Total number of assembled sequences |
11,864 |
| Number of contigs |
4,319 |
| True contigs (from >2 different clones) |
3,057 |
| Re-sequencing contigs† |
1,262 |
| Number of singletons |
7,545 |
| Number of putatively different fire ant sequences |
<11,864 |
| Average size of assembled sequences (bp) |
600.5 |
|
|
|
|
*High quality sequences are those with greater than 200 bp after trimming of vector and primer sequences and with a phred value higher than 15. In addition, this set excludes artifactual sequences that were manually removed. †Contigs composed of replicate sequences of only one clone |
|
|
Wang et al. Genome Biology 2007 8:R9 doi:10.1186/gb-2007-8-1-r9 |
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