Table 1

Fire ant EST and assembly statistics

Total number of sequence reads

28,133

cDNA clones sequenced from 5' end

22,560

Extra reads due to re-sequencing

5,573

High-quality sequences after filtering*

21,715

Average EST size after trimming (bp)

522.4

Total number of assembled sequences

11,864

Number of contigs

4,319

True contigs (from >2 different clones)

3,057

Re-sequencing contigs

1,262

Number of singletons

7,545

Number of putatively different fire ant sequences

<11,864

Average size of assembled sequences (bp)

600.5


*High quality sequences are those with greater than 200 bp after trimming of vector and primer sequences and with a phred value higher than 15. In addition, this set excludes artifactual sequences that were manually removed. Contigs composed of replicate sequences of only one clone

Wang et al. Genome Biology 2007 8:R9   doi:10.1186/gb-2007-8-1-r9

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