Table 1

Fire ant EST and assembly statistics

Total number of sequence reads

28,133

cDNA clones sequenced from 5' end

22,560

Extra reads due to re-sequencing

5,573

High-quality sequences after filtering*

21,715

Average EST size after trimming (bp)

522.4

Total number of assembled sequences

11,864

Number of contigs

4,319

True contigs (from >2 different clones)

3,057

Re-sequencing contigs^†

1,262

Number of singletons

7,545

Number of putatively different fire ant sequences

<11,864

Average size of assembled sequences (bp)

600.5

*High quality sequences are those with greater than 200 bp after trimming of vector and primer sequences and with a phred value higher than 15. In addition, this set excludes artifactual sequences that were manually removed. ^†Contigs composed of replicate sequences of only one clone

Wang et al. Genome Biology 2007 8:R9   doi:10.1186/gb-2007-8-1-r9