Table 3

Putative Hymenoptera-specific genes

Solenopsis invicta assembled sequence1

Blast statistics

Apis mellifera sequence

Confidence7


Identifier (length)

Span

Frame

ORF2 (bp)

I3

Exp4

Bit-score

E-value

Linkage Group

Span

Strand

ORF2 (bp)

Est5

Annotated gene6


SI.CL.8.cl.881.Contig1 (724 bp)

509-640

2

300

272

1.24E-18

6

2701427-2701558

+

429

Ab initio prediction

***


SI.CL.8.cl.843.SiJWH04BDO2.scf (730 bp)

582-761

3

147

210

1.99E-12

NW_001254419.8

44307-44486

-

147

Near NH homology. GB18184-PA on reverse strand

**


SI.CL.19.cl.1938.Contig1 (835 bp)

21-323

3

372

T

212

1.43E-12

6

1145090-1145392

-

429

Ab initio prediction. Near GB12791-PA on reverse strand

***


SI.CL.19.cl.1953.SiJWC11BBX.scf (613 bp)

81-215

3

555

166

5.08E-08

8

5253595-5253729

-

372

GB14543-PA. Near NH homology on reverse strand

*





306-416

87

4.5E-15

5252894-5253094

306





435-635

200

5253189-5253299

318


SI.CL.23.cl.2326.Contig1 (632 bp)

413-577

2

219

291

1.33E-20

11

8022183-8022347

+

480

Ab initio prediction

***


SI.CL.26.cl.2688.Contig1 (859 bp)

60-131

39

87

98

9.74E-15

9

10421877-10421948

-

549

Ab initio prediction. Near NH homology on reverse strand

**




119-256

29

558

186

10421751-10421888


SI.CL.33.cl.3311.Contig1 (710 bp)

228-359

3

189

258

3.07E-17

14

8634060-8634191

-

132

Near ab initio prediction. Near NH homology on reverse strand

*


SI.CL.33.cl.3384.Contig1 (469 bp)

229-327

19

264

T,S

160

3.11E-13

14

3770768-3770866

-

231

Ab initio prediction

***





362-454

29

180

S

104

3770649-3770741

186


SI.CL.35.cl.3595.Contig1 (415 bp)

123-398

3

342

301

5.97E-22

NW_001261806.8

12471-12746

+

327

Ab initio prediction

***


SiJWA02BAZ2.scf (600 bp)

374-469

2

261

193

2.13E-15

5

9909503-9909598

+

627

Near GB15931-PA and NH homology on reverse strand

*




533-604

98

9909356-9909427


SiJWA03CAW.scf (666 bp)

49-144

1

96

120

2.1E-16

NW_001259848.8

47860-47955

+

99

GB10007-PA on reverse strand

***






136-297

117

182

47704-47865

726


SiJWA12ACK.scf (212 bp)

137-268

29

69

264

1.42E-19

3

5151467-5151598

+

162

Near ab initio prediction and NH homology on reverse strand

**





63-143

39

72

69

5151391-5151471

189


SiJWB12BCQ.tag5_B12_04.scf (754 bp)

121-369

1

354

254

1.1E-16

7

5620128-5620376

+

336

Ab initio prediction on reverse strand

***


SiJWC11BAT.scf (342 bp)

189-278

3

228

160

3.98E-17

14

8645843-8645932

+

162

Near ab initio prediction and homology

**





282-368

123

6.41E-14

8645754-8645840

117


SiJWE02BBO2.scf (865 bp)

714-863

3

129

243

1.26E-15

6

4850974-4851123

-

354

Near ab initio prediction on reverse strand

**


SiJWF07BCC.tag5_F07_11.scf (799 bp)

329-529

2

96

196

6.59E-11

3

6205208-6205408

-

108

Near NH homology. Ab initio prediction on reverse strand

**


SiJWG01BDU2.scf (759 bp)

21-227

3

102

354

1.23E-26

2

9618145-9618351

+

171

GB12576-PA and NH homology on reverse strand

*


SiJWG03ACB.scf (623 bp)

172-609

1

471

558

4.63E-47

10

2344965-2345402

+

1440

GB19005-PA

***


SiJWH02AAN.scf (469 bp)

100-294

1

102

341

1.32E-30

12

281374-281568

-

294

-

***






28-105

69

104

281564-281641

207


SiJWH05BDPR5A08.scf (658 bp)

580-657

1

78

161

1.1E-15

10

2890267-2890344

+

159

Near ab initio prediction

**


SiJWH05BDV2.scf (517 bp)

204-353

3

198

237

4.87E-15

5

6704423-6704572

+

174

Ab initio prediction

**


SiJWH08AAT.scf (653 bp)

76-162

1

60

141

4.53E-20

5

1169177-1169263

+

84

Near ab initio prediction and NH homology

*






151-195

102

75

4.52E-13

1169261-1169305

69


SiJWH08ADY.scf (563 bp)

236-496

2

327

312

1.32E-22

12

4477772-4478032

-

432

GB16574-PA

***


1Solenopsis invicta assembled sequences that show no significant similarity to any known non-hymenopteran sequence (E > 1), but high similarity to a region of the honey bee genome (E < e-10). 2Length in base-pairs of the largest overlapping in-frame open reading frame. 3In-frame Interproscan annotation of fire ant assembled sequence. T means 'transmembrane region', S means 'signal peptide'. 4Gene is known (•) to be expressed in fire ant (unpublished microarray data). 5In honey bee, EST evidence exists (•) within 5,000 bp of the aligned region. 6This column shows the annotation of overlapping or nearby (within 5,000 bp) honey bee genes, as well as the nearby presence of genes from non-hymenopteran organisms. Numbers starting with GB are honeybee Official Gene Set numbers. 'Ab initio prediction' indicates that Gnomon, Genscan, or another algorithm was used to predict a gene that was not retained for the bee genome Official Gene Set. 'NH homology' indicates the nearby presence of a gene from non-hymenopteran organisms. 7Based on visual inspection we assigned a confidence level (the more asterisks the better) to each ant-bee putative gene pair (see Materials and methods). 8Apis mellifera unanchored scaffolds such as NW_001254419.1 are regions that have not been mapped to a chromosome. 9Multiple alignment frames for a S. invicta transcript indicate possible frameshifts during sequencing.

Wang et al. Genome Biology 2007 8:R9   doi:10.1186/gb-2007-8-1-r9

Open Data