Research
Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyte
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* Corresponding author: Ueli Grossniklaus grossnik@botinst.uzh.ch
- † Equal contributors
1 Institute of Plant Biology and Zürich-Basel Plant Science Center, Zollikerstrasse, University of Zürich, CH-8008 Zürich, Switzerland
2 Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, PT-2780-156 Oeiras, Portugal
3 Current address: Institute of Plant Sciences and Zürich-Basel Plant Science Center, ETH Zürich, Universitätstrasse, CH-8092 Zürich, Switzerland
Genome Biology 2007, 8:R204 doi:10.1186/gb-2007-8-10-r204
Published: 3 October 2007Additional files
Additional data file 1:
Presented is a table listing the gene validation for embryo sac expression.
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Additional data file 2:
Presented is a list of the identity of embryo sac expressed genes, as revealed by genetic subtraction of coa from the wild type.
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Additional data file 3:
Presented is a list of embryo sac expressed genes, identified by a reanalysis of the previously published dataset using the spl mutant [34].
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Additional data file 4:
Presented is a list of genes from this work that were previously identified as being essential for reproductive development.
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Additional data file 5:
Presented is a list of those genes that were found to be over-expressed in the carpel and ovule tissues of coa and spl in the absence of an embryo sac.
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Additional data file 6:
Illustrated is the scale of gene discovery by three independent methods across two types of datasets from two mutants.
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Additional data file 7:
Presented is a table summarizing gene identities and the statistical treatments, confirming the necessity of different statistical treatments to identify embryo sac expressed genes.
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Additional data file 8:
Listed are the identifiers of maize and wheat ESTs from the embryo sac cell types, which were used in BLAST analysis of Arabidopsis proteins.
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Additional data file 9:
Provided are details of previously reported transcriptome datasets used in data comparison.
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Additional data file 10:
Described is the methodology employed for transcriptional profiling by oligonucleotide array.
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Additional data file 11:
Listed are the primers used for mutant genotyping, probes for mRNA in situ hybridization and RT-PCR.
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