  ResearchGenetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female gametophyte and the maternal sporophyteAmal J Johnston* 1,3 1Institute of Plant Biology and Zürich-Basel Plant Science Center, Zollikerstrasse, University of Zürich, CH-8008 Zürich, Switzerland 2Centro de Biologia do Desenvolvimento, Instituto Gulbenkian de Ciência, Rua da Quinta Grande, PT-2780-156 Oeiras, Portugal 3Current address: Institute of Plant Sciences and Zürich-Basel Plant Science Center, ETH Zürich, Universitätstrasse, CH-8092 Zürich, Switzerland
Genome Biology 2007, 8:R204doi:10.1186/gb-2007-8-10-r204
Subject areas: Plant biology, Development, Genome studies Additional filesAdditional data file 1: Presented is a table listing the gene validation for embryo sac expression. Format: PDF Size: 100KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 2: Presented is a list of the identity of embryo sac expressed genes, as revealed by genetic subtraction of coa from the wild type. Format: XLS Size: 138KB Download file This file can be viewed with: Microsoft Excel Viewer Additional data file 3: Presented is a list of embryo sac expressed genes, identified by a reanalysis of the previously published dataset using the spl mutant [34]. Format: XLS Size: 253KB Download file This file can be viewed with: Microsoft Excel Viewer Additional data file 4: Presented is a list of genes from this work that were previously identified as being essential for reproductive development. Format: PDF Size: 105KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 5: Presented is a list of those genes that were found to be over-expressed in the carpel and ovule tissues of coa and spl in the absence of an embryo sac. Format: XLS Size: 269KB Download file This file can be viewed with: Microsoft Excel Viewer Additional data file 6: Illustrated is the scale of gene discovery by three independent methods across two types of datasets from two mutants. Format: PDF Size: 41KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 7: Presented is a table summarizing gene identities and the statistical treatments, confirming the necessity of different statistical treatments to identify embryo sac expressed genes. Format: PDF Size: 15KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 8: Listed are the identifiers of maize and wheat ESTs from the embryo sac cell types, which were used in BLAST analysis of Arabidopsis proteins. Format: PDF Size: 71KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 9: Provided are details of previously reported transcriptome datasets used in data comparison. Format: PDF Size: 18KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 10: Described is the methodology employed for transcriptional profiling by oligonucleotide array. Format: PDF Size: 26KB Download file This file can be viewed with: Adobe Acrobat Reader Additional data file 11: Listed are the primers used for mutant genotyping, probes for mRNA in situ hybridization and RT-PCR. Format: PDF Size: 13KB Download file This file can be viewed with: Adobe Acrobat Reader |
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