Research
Divergent evolution of arrested development in the dauer stage of Caenorhabditis elegans and the infective stage of Heterodera glycines
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* Corresponding author: Thomas J Baum tbaum@iastate.edu
1 Interdepartmental Genetics Program, Iowa State University, Ames, IA 50011, USA
2 Department of Plant Pathology, Iowa State University, Ames, IA 50011, USA
3 Current address: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
4 Department of Genetics, Washington University School of Medicine, Genome Sequencing Center, St Louis, MO 63108, USA
5 Department of Statistics, Iowa State University, Ames, IA 50011, USA
6 LH Baker Center for Bioinformatics and Biological Statistics, Iowa State University, Ames, IA 50011, USA
7 Current address: Center for Biomedical Informatics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
8 Current address: The University of Kansas Medical Center, Kansas City, KS 66160, USA
9 Department of Plant Pathology, NC State University, Raleigh, NC 27695, USA
10 Department of Plant Pathology, University of Georgia, Athens, GA 30602, USA
11 Divergence Inc., North Warson Road, St Louis, MO 63141, USA
Genome Biology 2007, 8:R211 doi:10.1186/gb-2007-8-10-r211
Published: 5 October 2007Additional files
Additional data file 1:
BLAST searches identified seven H. glycines probesets orthologous to C. elegans FaRP-encoding genes (mean expression pattern indicated in red). For visualization purposes, each probeset's estimated mean log-scale expression profile was standardized to have mean 0 and variance 1.5 prior to plotting. infJ2, infective J2; parJ2, parasitic J2.
Format: EPS Size: 7KB Download file
Additional data file 2:
Reciprocal BLAST searches identified 438 H. glycines probesets as orthologous to C. elegans dauer-regulated genes. These probesets were grouped into nine clusters based on their temporal expression profiles. The average expression pattern of the probesets in each cluster is indicated by a bold line. infJ2, infective J2; parJ2, parasitic J2.
Format: EPS Size: 27.4MB Download file
Additional data file 3:
The 25 most conserved H. glycines contigs compared to C. elegans.
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Additional data file 4:
Identities and cluster membership of differentially expressed (FDR 5%) probesets over the entire life cycle.
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Additional data file 5:
The 25 most abundant InterPro domains for the 10 expression clusters for H. glycines genes that are differentially expressed over the entire life cycle.
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Additional data file 6:
H. glycines probesets orthologous to dauer-regulated C. elegans genes and their cluster memberships.
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Additional data file 7:
qRT-PCR results, oligonucleotide primers used and probeset identities.
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Additional data file 8:
Transformed and normalized Affymetrix probeset mean data for each probeset in each life stage and q values for each tested combination of life stages as described in Materials and methods.
Format: CSV Size: 2.5MB Download file