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Open Access Research

Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease

Griet Vanpoucke1, Brigid Orr1, O Cathal Grace1, Ray Chan1, George R Ashley1, Karin Williams2, Omar E Franco2, Simon W Hayward2 and Axel A Thomson1*

Author Affiliations

1 MRC Human Reproductive Sciences Unit, The Queens Medical Research Institute, Little France Crescent, Edinburgh EH16 4TJ, UK

2 Departments of Urologic Surgery and Cancer Biology, Vanderbilt University Medical Center, 21st Avenue South, Nashville, TN 37232-2765, USA

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Genome Biology 2007, 8:R213  doi:10.1186/gb-2007-8-10-r213

Published: 8 October 2007

Abstract

Background

The mesenchymal compartment plays a key role in organogenesis, and cells within the mesenchyme/stroma are a source of potent molecules that control epithelia during development and tumorigenesis. We used serial analysis of gene expression (SAGE) to profile a key subset of prostatic mesenchyme that regulates prostate development and is enriched for growth-regulatory molecules.

Results

SAGE libraries were constructed from prostatic inductive mesenchyme and from the complete prostatic rudiment (including inductive mesenchyme, epithelium, and smooth muscle). By comparing these two SAGE libraries, we generated a list of 219 transcripts that were enriched or specific to inductive mesenchyme and that may act as mesenchymal regulators of organogenesis and tumorigenesis. We identified Scube1 as enriched in inductive mesenchyme from the list of 219 transcripts; also, quantitative RT-PCR and whole-mount in situ hybridization revealed Scube1 to exhibit a highly restricted expression pattern. The expression of Scube1 in a subset of mesenchymal cells suggests a role in prostatic induction and branching morphogenesis. Additionally, Scube1 transcripts were expressed in prostate cancer stromal cells, and were less abundant in cancer associated fibroblasts relative to matched normal prostate fibroblasts.

Conclusion

The use of a precisely defined subset of cells and a back-comparison approach allowed us to identify rare mRNAs that could be overlooked using other approaches. We propose that Scube1 encodes a novel stromal molecule that is involved in prostate development and tumorigenesis.