Table 10 |
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Identification of the genetic determinants for host specificity of Xcc 8004 on certain plants |
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Candidate avr genes in Xcc 8004 |
Mutational analysis† |
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|
|
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Postulated avr gene |
Gene ID |
Annotated function |
Correlation (CC)* |
Mutant ID |
Virulence |
8004 |
Plants |
|
|
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|
avrRc1 |
XC2004 |
Avirulence protein, avrXccC |
-1 |
NK2004 |
+ |
- |
TP1 |
|
XC2084 |
Tn5041 transposase |
-1 |
... |
... |
... |
... |
|
|
avrRc3 |
XC2602 |
Avirulence protein, avrXccE1 |
-0.88 |
NK2602 |
+ |
- |
TP8 |
|
avrRp1 |
XC2081 |
Avirulence protein, avrBs1 gene |
1 |
NK2081 |
N |
HR |
TP12 |
|
|
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*The direct correlation analysis between virulence of the 20 Xcc strains on one given plant cultivar and the distribution status of each gene in 20 Xcc strains was carried out using the program CORREL. The maximal absolute CC values were selected from Additional data file 8. †The mutants of the candidate genes were generated by means of pK18mob integration and the plant tests were carried out on given plants that showed specific reactions to certain Xcc strains. TP1, mustard (B. juncea var. megarrhiza Tsen et Lee) cv. Guangtou; TP8, Chinese cabbage (B. rapa subsp. pekinensis) cv. Zhongbai-83; TP12, pepper (Capsicum annuum v. latum) ECW10R. +, virulent; -, non-pathogenic. The hypersensitive reaction (HR) tests of Xcc strains were carried out on TP12. HR, positive HR result; N, no HR. ..., data unavailable. |
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He et al. Genome Biology 2007 8:R218 doi:10.1186/gb-2007-8-10-r218 |
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