Figure 2.

Comparison of transcript abundance quantified by microarray analysis and quantitative RT-PCR (qpcr). The changes in mRNA levels of six genes following UV irradiation were analyzed by quantitative RT-PCR to provide an independent confirmation of the microarray data. The log₂ expression values for ssb (closed triangles), cdc6-2 (closed circles), tfb1 (closed squares), tfb2 (open squares), tfb3 (open circles) and dpoII (open triangles) obtained from microarray and RT-PCR analyses are plotted on the x and y axes, respectively. The data obtained from the two methods yield a linear fit with a slope of 0.93 and R-value of 0.91.