Table 2

Summary of data from quantitative RT-PCR on the S. solfataricus genes tfb3, cdc6-2, xpb1, dpoII and ssb


CP*
 †UV/control expression ratio


Protein
 Sso no.
 UV (120 min)
 Control (120 min)
 30 min
 60 min
 90 min
 120 min
TFB1
 0446
 14.7 ± 0.21
 14.5 ± 0.06
 0.76
 1.02
 1.37
 0.92
TFB2
 0946
 21.4 ± 0.21
 20.2 ± 0.15
 0.71
 0.54
 0.83
 0.49
TFB3
 0280
 12.5 ± 0.06
 16.3 ± 0.06
 2.2
 14.4
 24.2
 12.6
Cdc6-2
 0771
 12.7 ± 0.06
 15.8 ± 0
 1.8
 7.4
 6.6
 4.7
XPB1
 0959
 13.8 ± 0.1
 13.6 ± 0.12
 0.80
 1.07
 1.05
 0.92
DpoII
 1459
 11.1 ± 0.12
 13.7 ± 0.06
 1.0
 3.07
 4.41
 3.44
SSB
 2364
 9.9 ± 0.12
 9.8 ± 0.23
 0.98
 0.88
 0.92
 0.90

*CP: the cycle number at which the fluorescence crossed the threshold during PCR. The lower the CP, the more abundant the transcript. CP values are means of triplicate experiments with standard deviations shown. †The expression ratio was calculated according to the method of [50].

Götz et al. Genome Biology 2007 8:R220   doi:10.1186/gb-2007-8-10-r220

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