Table 2 |
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|
Main genes regulated by Hfe deficiency in liver and pertaining to enriched functional categories related to response to oxidative stress |
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| Gene |
Protein |
S-score |
||
|
|
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| D2 KO versus WT |
B6 KO versus WT |
D2 WT versus B6 WT |
||
|
|
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| Glutathione metabolism pathway |
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| Gclc |
Glutamate-cysteine ligase, catalytic subunit |
3.68 |
4.74 |
3.24 |
| Gclm |
Glutamate-cysteine ligase, modifier subunit |
NS |
4.11 |
NS |
| Gss |
Glutathione synthetase |
NS |
2.15 |
NS |
| Gsta2 |
Glutathione S-transferase alpha2 |
8.83 |
9.10 |
NS |
| Gsta3 |
Glutathione S-transferase alpha3 |
2.44 |
2.24 |
NS |
| Gsta4 |
Glutathione S-transferase alpha4 |
4.64 |
5.99 |
4.35 |
| Gstm1 |
Glutathione S-transferase mu1 |
NS |
2.65 |
NS |
| Gstm3 |
Glutathione S-transferase mu3 |
3.04 |
4.04 |
2.56 |
| Gstm6 |
Glutathione S-transferase mu6 |
3.36 |
2.26 |
NS |
| UDP glucuronosyltransferase activity |
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| Ugt2b1 |
UDP glucuronosyltransferase 2B1 |
3.72 |
NS |
NS |
| Ugt2b5 |
UDP glucuronosyltransferase 2B5 |
2.56 |
3.91 |
NS |
| Ugt2b34 |
UDP glucuronosyltransferase 2B34 |
NS |
2.25 |
NS |
| Ugt2b35 |
UDP glucuronosyltransferase 2B35 |
2.56 |
3.91 |
NS |
| Ugt2b36 |
UDP glucuronosyltransferase 2B36 |
4.66 |
NS |
-4.89 |
| Mono-oxygenase activity |
||||
| Cyp1a2 |
Cytochrome P450 1A2 |
3.14 |
2.60 |
3.49 |
| Cyp2c29 |
Cytochrome P450 2C29 |
NS |
2.79 |
4.84 |
| Cup2c44 |
Cytochrome P450 2C44 |
3.07 |
NS |
-5.89 |
| Cyp2c55 |
Cytochrome P450 2C55 |
3.94 |
6.22 |
4.43 |
| Cyp2c70 |
Cytochrome P450 2C70 |
5.55 |
4.70 |
4.17 |
| Cyp2j6 |
Cytochrome P450 2J6 |
2.70 |
NS |
NS |
| Cyp2j9 |
Cytochrome P450 2J9 |
NSD |
2.91 |
2.14 |
| Cyp2u1 |
Cytochrome P450 2U1 |
3.50 |
NS |
NS |
| Fmo5 |
Flavin mono-oxygenase 5 |
2.12 |
NS |
NS |
| Iron ion binding |
||||
| Ftl1 |
Ferritin light chain 1 |
1.70 |
2.24 |
NS |
| Slc40a1 |
Ferroportin |
3.18 |
4.89 |
3.94 |
| Hmox1 |
Heme oxygenase 1 |
5.27 |
2.40 |
-3.66 |
| Blvrb |
Biliverdin reductase (for information) |
2.50 |
3.06 |
NS |
| Vnn1 |
Vanin 1 (for information) |
-4.27 |
-2.73 |
2.69 |
|
|
||||
|
S-scores were obtained as described in Materials and methods and are proportional to fold changes. Positive S-scores indicate that the genes are more highly expressed in knockout (KO) than in wild-type (WT) mice, or in WT D2 than in WT B6 mice. NS, not significant; UDP, Uridine 5'-diphospho. |
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|
Coppin et al. Genome Biology 2007 8:R221 doi:10.1186/gb-2007-8-10-r221 |
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