Table 5

Changes in expression of genes involved in iron metabolism
Gene
 Protein
 Major biochemical activity
 Role
 Organ
 S-score










D2 KO versus D2 WT
 B6 KO versus B6 WT
 D2 WT versus B6 WT
Iron storage
     Ftl1
 Ferritin L chain
 Fe mineralization
 Cytosolic storage
 Liver
 +1.70
 +2.24
 NS
Iron transport
     Slc40a1
 Ferroportin
 Membrane transporter
 Cellular export
 Liver
 +3.18
 +4.89
 +3.94
     Abcg2
 Bcrp
 Membrane transporter
 Possible mitochondrial heme export
 Liver
 +2.97
 NS
 NS
     Sfxn2
 Sideroflexin2
 Membrane transporter
 Mitochondrial traffic
 Liver
 -2.38
 -2.16
 NS
     Slc25a37
 Mitoferrin
 Membrane transporter
 Mitochondrial traffic
 Liver
 NS
 +2.28
 NS
     Lcn2
 Lipocalin2
 Siderophore iron binding
 Traffic of siderophore-bound iron
 Liver
 -2.91
 NS
 NS
Receptors
     Tfrc
 Transferrin receptor1
 Transferrin binding
 Transferrin iron uptake
 Duodenum
 -2.07
 NS
 NS
     Lrp1
 LRP/CD91
 Hemoplexin receptor
 Hemoplexin uptake
 Liver
 NS
 -2.03
 NS
Regulators
     Ireb2
 IRP2
 RNA binding
 Control of cellular iron
 Duodenum
 +2.26
 NS
 -2.64
     Hamp1
 Hepcidin 1
 Ferroportin binding
 Control of systemic iron
 Liver
 -6.27
 NS
 -3.57
     Hamp2
 Hepcidin 2
 ?
 ?
 Liver
 -3.40
 NS
 +3.36
     Hfe
 HFE
 TfR1 binding
 ?
 Liver
 -7.96
 -8.96
 -3.16




Duodenum
 -5.70
 -7.32
 NS
     Hfe2
 HJV
 Neogenin binding
 Control of hepcidin expression
 Liver
 NS
 -2.01
 NS
     Fxn
 Frataxin
 Iron binding
 Chaperon for Fe-S synthesis
 Liver
 NS
 NS
 -3.09
     Smad4
 Smad4
 Transcription factor
 Control of hepcidin expression
 Liver
 NS
 NS
 -3.59




Duodenum
 NS
 NS
 -5.05
     Usf2
 Usf2
 Transcription factor
 Control of hepcidin expression
 Liver
 NS
 +2.08
 NS
Oxidoreductases
     Cybrd1
 Dcytb
 Fe(III) reduction
 Facilitates duodenal transport by DMT1
 Duodenum
 +2.97
 NS
 NS

Iron metabolism genes are cited in this table where significant expression variations in Hfe-/- mice (knockout [KO]) or expression differences between wild-type (WT) strains were detected. S-scores were obtained as described in Material and Methods and are proportional to fold changes. Positive S-scores indicate that the genes are more highly expressed in KO than in WT mice, or in WT D2 than in WT B6 mice. NS, not significant.

Coppin et al. Genome Biology 2007 8:R221   doi:10.1186/gb-2007-8-10-r221

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