Table 8 |
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|
Sequences of the primers used for validation of microarray results by real-time PCR |
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|
Gene |
GeneBank accession |
Forward primer |
Reverse primer |
Amplification efficiency |
|
|
||||
|
Hprt |
5'-CTG GTT AAG CAG TAC AGC CCC AA-3' |
5'-CAG GAG GTC CTT TTC ACC AGC-3' |
1.99 |
|
|
Aox1 |
5'-CAC CCT GTA TTC ATC TAA GGG CCT-3' |
5'-CAC TGC ATC ATG GAT GGC AA-3' |
1.92 |
|
|
Ftl1 |
5'-GGA GAA GAA CCT GAA TCA GGC C-3' |
5'-GGT TGC CCA TCT TCT TGA TGA G-3' |
2.00 |
|
|
Fpn1 |
5'-CAT TGC TGC TAG AAT CGG TCT T-3' |
5'-GCA ACT GTG TCA CCG TCA AAT-3' |
1.97 |
|
|
Hmox1 |
5'-CAG ATG GCG TCA CTT CGT CA-3' |
5'-CTC TGC AGG GGC AGT ATC TTG-3' |
2.00 |
|
|
Vanin1 |
5'-GGC TGC ACA CCG TGG AAG-3' |
5'-GGT AAA AGC CGT GTC CAC TGA A-3' |
1.90 |
|
|
Por |
5'-GCC TCG TCG TCT AAG GTC CA-3' |
5'-GAC TTC GCT TCA TAC TCC ACA GC-3' |
1.99 |
|
|
Cpt1a |
5'-GAC CCC ACA ACA ACG GCA G-3' |
5'-ATG GCG AGG CGG TAC AGG T-3' |
2.00 |
|
|
Aco2 |
5'-GAC CAT TCA AGG CCT GAA GG-3' |
5'-ACG CAC TTC AGA GGC TTT CC-3' |
2.00 |
|
|
Cyp7a1 |
5'-GCT CTG GAG GGA ATG CCA T-3' |
5'-CCG CAG AGC CTC CTT GAT G-3' |
2.00 |
|
|
Hsd3b5 |
5'-AGA GGA ATT GTC CAA GCT GCA-3' |
5'-TGT GGA TGA CAG CAG ACA TGC-3' |
1.99 |
|
|
Hfe2 |
5'-ACC ACC ATC CGG AAG ATC ACT-3' |
5'-AAG GCT GCA GGA AGA TTG TCC-3' |
2.00 |
|
|
Hamp1 |
5'-AAG CAG GGC AGA CAT TGC GAT-3' |
5'-CAG GAT GTG GCT CTA GGC TAT GT-3' |
1.98 |
|
|
Hamp2 |
5'-AAG CAG GGC AGA CAT TGC GAT-3' |
5'-GGA TGT GGC TCT AGG CTC TCT ATT-3' |
2.00 |
|
|
Usf2 |
5'-ATG GAA CCA GAA CTC CTC GAG A-3' |
5'-CCG TTC CAC TTC ATT GTG CTG-3' |
1.93 |
|
|
Lcn2 |
5'-TCT GTC CCC ACC GAC CAA T-3' |
5'-CCA GTC AGC CAC ACT CAC CAC-3' |
1.99 |
|
|
Sfxn2 |
5'-CGC ACA AGT GGT TAT CTC TCG G-3' |
5'-CCA TGA TGA CAG GCA ACA GGA-3' |
1.99 |
|
|
Alas2 |
5'-TGG AAC TCT TGG CAA GGC C-3' |
5'-CAA GTC CCG AGT GCT GGC T-3' |
1.99 |
|
|
Slc25a37 |
5'-GAG CAC TCC ATC ATG TAC CCG-3' |
5'-TGG ATT CAA ACT CTG CAT CCG-3' |
2.00 |
|
|
Abcg2 |
5'-TTG GCT GTC CTG GCT TCA GTA C-3' |
5'-CAA AGC TGT GAA GCC ATA TCG A-3' |
1.99 |
|
|
Cybrd1 |
5'-GCA GCG GGC TCG AGT TTA-3' |
5'-TTC CAG GTC CAT GGC AGT CT-3' |
1.98 |
|
|
Slc39a4 |
5'-GCG ACT GAG AGC AGA GCT GA-3' |
5'-GTT GTG TAC CGC GTC GCC-3' |
2.00 |
|
|
Mucin3 |
5'-TCG TGT TCT CCA TCC GCT TC-3' |
5'-GAC ACT CTG GAC CGT TGC CT-3' |
1.99 |
|
|
Lcn13 |
5'-TGT TTG TGC CAG AGA TCG GAG-3' |
5'-GCT GGC TCA GCT GTT GCA G-3' |
1.95 |
|
|
Fmo3 |
5'-GGA ACT TGC ACT TTG CCT TCT G-3' |
5'-GGT GGT GCT ATT GCC ATA CCA-3' |
1.96 |
|
|
Clca4 |
5'-GCC GTC ATA GAA GCT GAG AGT GG-3' |
5'-AGC ACC TGC CCC GTT GTC-3' |
2.00 |
|
|
|
||||
|
Hfe-/- and Hfe+/+ mice (five males per group) were killed at age 7 weeks. Blood was removed and plasma lipid levels were determined by chromatography. Results are expressed as mean ± standard deviation in each group. P values for comparisons of plasma lipid levels between Hfe-/- and Hfe+/+ mice of each strain were obtained by Student's t-test. HDL, high-density lipoprotein. |
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|
Coppin et al. Genome Biology 2007 8:R221 doi:10.1186/gb-2007-8-10-r221 |
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