|
Characterization of hESCs and BCs by immunocytochemistry and Affymetrix arrays |
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| Level I |
Level II |
Level III |
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|
|
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| IC |
BC direct |
Epithelium |
Leukocytes |
Endothelium |
Stromal |
|
|
|
||||||
| GATA1 |
+ |
+ |
+ |
+ |
+ |
+ |
| GATA2 |
+ |
+ |
+ |
-- |
-- |
-- |
| CD71 |
+ |
+ |
+ |
+ |
-- |
+ |
| EPO-R |
+ |
-- |
+ |
+ |
+ |
+ |
| TPO-R |
+ |
-- |
-- |
-- |
-- |
+ |
| LMO2 |
+ |
+ |
+ |
+ |
-- |
+ |
| β-Catenin |
+ |
-- |
-- |
-- |
-- |
-- |
| EGR1 |
+ |
-- |
-- |
-- |
-- |
-- |
| Integrin α4 |
+/- |
-- |
-- |
-- |
-- |
-- |
| Integrin β1 |
+/- |
-- |
-- |
-- |
-- |
-- |
| VE-cadherin |
-- |
-- |
-- |
+ |
-- |
-- |
| E-cadherin |
-- |
-- |
-- |
-- |
-- |
-- |
| KDR |
-- |
-- |
-- |
-- |
-- |
-- |
| PECAM (CD31) |
-- |
-- |
-- |
+ |
-- |
-- |
| CD34 |
-- |
-- |
-- |
+ |
-- |
-- |
| CD41 |
-- |
-- |
CD41b |
-- |
CD41b |
CD41b |
| CD43 |
-- |
-- |
-- |
+ |
+ |
+ |
|
The reliability of the microarray data generated from our multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which a subset of genes were confirmed by immunocytochemistry. For immunochemistry (IC): +, moderate to strong staining; -, negative staining; +/-, very weak staining. For Affymetrix arrays:+, detected as up-regulated in BCs; --, not detected as up-regulated in BCs. | ||||||
Lu et al. Genome Biology 2007 8:R240 doi:10.1186/gb-2007-8-11-r240 |
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