Table 7 |
|||||
|
Primer sequences |
|||||
|
Gene |
Primer |
Sequence |
Annealing Tm (C) |
MgCl2 conc. (μM) |
Product size (bp) |
|
|
|||||
|
HPRT |
Sense Antisense |
5'-CTTGCGACCTTGACCATCTTTGGA-3' 5'-GGCGTCGTGATTAGTGATGATGAACC-3' |
58 |
1.5 |
467 |
|
GATA-2 |
Sense Antisense |
5'-TATGTGCCGGCGGCTGCCCACGACTACA-3' 5'-GGCTCTTCTGGCGGCCGACAGTCTT-3' |
60 |
1.5 |
280 |
|
SCL |
Sense Antisense |
5'-GAAGTGCTCCCCTCTGAAAGTT-3' 5'-GGCTATCTCTCCTCTGACCTCG-3' |
58 |
1.5 |
319 |
|
β-Cluster globin genes |
Sense Antisense |
5'-GTYTACCCHTGGACCCAGA-3' 5'-GCAGCTTGTCACAGTGCAG-3' |
56 |
2 |
190 |
|
OCT4 |
Sense Antisense |
5'-GAAGGTATTCAGCCAAACGAC-3' 5'-GTTACAGAACCACACTCGGA-3' |
55 |
2 |
315 |
|
Nanog |
Sense Antisense |
5'-TGCAAATGTCTTCTGCTGAGAT-3' 5'-GTTCAGGATGTTGGAGAGTTC-3' |
55 |
2 |
285 |
|
Rex-1 |
Sense Antisense |
5'-TGACAGGCAAGAAGCTTCCG-3' 5'-GCGTACGCAAATTAAAGTCCAGA-3' |
55 |
2 |
350 |
|
|
|||||
|
Lu et al. Genome Biology 2007 8:R240 doi:10.1186/gb-2007-8-11-r240 |
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