Method
Application of phage display to high throughput antibody generation and characterization
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* Corresponding author: John McCafferty jm635@cam.ac.uk
1 Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK
2 Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1HH, UK
3 Cambridge Antibody Technology, Granta Park, Cambridge CB21 6GH, UK
4 Cancer Research UK, Research Monoclonal Antibody Services, Lincoln's Inn Fields, London WC2A 3PX, UK
5 Molecular Products Ltd, Thaxted CM6 2LT, UK
6 Department of Biochemistry, University of Cambridge, Downing Site, Cambridge CB2 1QW, UK
7 Cellular Histopathology Department, Bedford Hospital NHS Trust, Bedford MK42 9DJ, UK
8 Cancer Research UK, Cambridge Research Institute, Cambridge CB2 0RE, UK
9 GlaxoSmithKline Medicines Research Center, Stevenage SG1 2NY, UK
10 Genzyme Therapeutics Ltd, Cambridge Science Park, Cambridge CB4 0WG, UK
11 Novartis Institutes for BioMedical Research, Discovery Technologies, CH-4002 Basel, Switzerland
12 Astra Zeneca Innovation Centre China, Shanghai, 200041, China
Genome Biology 2007, 8:R254 doi:10.1186/gb-2007-8-11-r254
Published: 29 November 2007Additional files
Additional data file 1:
All 406 antigens used in antibody selection and the number of unique antibodies generated.
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Additional data file 2:
Median fluorescent intensities of antigen beads as plotted in Figure 5.
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Additional data file 3:
Description of staining profiles supported by multiple antibodies in immunohistochemistry.
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Additional data file 4:
Construction and rescue of the 'McCafferty' antibody phage display library
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