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Open Access Method

Direct selection and phage display of a Gram-positive secretome

Dragana Jankovic12, Michael A Collett2, Mark W Lubbers3 and Jasna Rakonjac1*

Author Affiliations

1 Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand

2 Fonterra Research Centre, Palmerston North, New Zealand

3 Fonterra, Mount Waverley, VIC 3149, Australia

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Genome Biology 2007, 8:R266  doi:10.1186/gb-2007-8-12-r266

Published: 13 December 2007

Abstract

Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.