Method

Direct selection and phage display of a Gram-positive secretome

Dragana Jankovic^1,2 , Michael A Collett² , Mark W Lubbers³ and Jasna Rakonjac¹

¹Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand

²Fonterra Research Centre, Palmerston North, New Zealand

³Fonterra, Mount Waverley, VIC 3149, Australia

author email corresponding author email

Genome Biology 2007, 8:R266doi:10.1186/gb-2007-8-12-r266

Published:	13 December 2007

Subject areas: Genome studies, Microbiology and parasitology

Abstract

Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.