Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity

Sara Holland¹ , Emma Lodwig¹ , Theodora Sideri¹ , Tom Reader¹ , Ian Clarke² , Konstantinos Gkargkas³ , David C Hoyle² , Daniela Delneri⁴ , Stephen G Oliver³ and Simon V Avery¹

¹School of Biology, Institute of Genetics, The University of Nottingham, University Park, Nottingham NG7 2RD, UK

²North West Institute for Bio-Health Informatics, The University of Manchester, ISBE, School of Medicine, Oxford Road, Manchester M13 9PT, UK

³Department of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge CB2 1GA, UK

⁴Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, UK

author email corresponding author email

Genome Biology 2007, 8:R268doi:10.1186/gb-2007-8-12-r268


Published:	18 December 2007

Subject areas: Genetics, Genome studies, Molecular biology, Physiology

Additional files

Additional data file 1:

Growth data for each heterozygote strain under Cr stress.

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Additional data file 2:

Over-represented GO terms among genes that gave significant haploinsufficiency or haploproficiency.

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Additional data file 3:

Exponential-phase cells were sub-cultured, in 300 μl volumes of YNB in 48-well plates, and growth (OD₆₀₀) was subsequently monitored at 30°C with continuous shaking in a plate reader. Doubling times were determined during the period of exponential growth in the absence or presence of 0.1 mM CrO₃, and the percent increase in doubling time attributable to Cr (a measure of Cr sensitivity) was calculated for each strain. The values are the means of three independent experiments ± standard error of the mean.

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Additional data file 4:

Affymetrix data for each heterozygote strain under the control and Cr conditions

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Additional data file 5:

Log.-ratio data for each heterozygote strain under the control and Cr conditions

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