Research
Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity
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* Corresponding author: Simon V Avery Simon.Avery@nottingham.ac.uk
1 School of Biology, Institute of Genetics, The University of Nottingham, University Park, Nottingham NG7 2RD, UK
2 North West Institute for Bio-Health Informatics, The University of Manchester, ISBE, School of Medicine, Oxford Road, Manchester M13 9PT, UK
3 Department of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge CB2 1GA, UK
4 Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester M13 9PT, UK
Genome Biology 2007, 8:R268 doi:10.1186/gb-2007-8-12-r268
Published: 18 December 2007Additional files
Additional data file 1:
Growth data for each heterozygote strain under Cr stress.
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Additional data file 2:
Over-represented GO terms among genes that gave significant haploinsufficiency or haploproficiency.
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Additional data file 3:
Exponential-phase cells were sub-cultured, in 300 μl volumes of YNB in 48-well plates, and growth (OD600) was subsequently monitored at 30°C with continuous shaking in a plate reader. Doubling times were determined during the period of exponential growth in the absence or presence of 0.1 mM CrO3, and the percent increase in doubling time attributable to Cr (a measure of Cr sensitivity) was calculated for each strain. The values are the means of three independent experiments ± standard error of the mean.
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Additional data file 4:
Affymetrix data for each heterozygote strain under the control and Cr conditions
Format: XLS Size: 5.6MB Download file
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Additional data file 5:
Log.-ratio data for each heterozygote strain under the control and Cr conditions
Format: XLS Size: 2.1MB Download file
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