Genome Biology

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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

Jan Hellemans, Geert Mortier, Anne De Paepe, Frank Speleman and Jo Vandesompele*

Genome Biology 2007, 8:R19 doi:10.1186/gb-2007-8-2-r19

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Research article   Open Access

Identification of differentially expressed genes in SHSY5Y cells exposed to okadaic acid by suppression subtractive hybridization

Vanessa Valdiglesias, Juan Fernandez-Tajes, Eduardo Pasaro, Josefina Mendez, Blanca Laffon BMC Genomics 2012, 13:46 (27 January 2012)

Research article   Open Access

Genome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genes

Kate E Killick, John A Browne, Stephen DE Park, David A Magee, Irene Martin, Kieran G Meade, Stephen V Gordon, Eamonn Gormley, Cliona O'Farrelly, Karsten Hokamp, David E MacHugh BMC Genomics 2011, 12:611 (19 December 2011)

Research article   Open Access

SND2, a NAC transcription factor gene, regulates genes involved in secondary cell wall development in Arabidopsis fibres and increases fibre cell area in Eucalyptus

Steven G Hussey, Eshchar Mizrachi, Antanas V Spokevicius, Gerd Bossinger, Dave K Berger, Alexander A Myburg BMC Plant Biology 2011, 11:173 (1 December 2011)

Research article   Open Access

Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida)

Maik Klie, Thomas Debener BMC Research Notes 2011, 4:518 (28 November 2011)

Research article   Open Access

Evaluation of glyceraldehyde-3-phosphate, prolylpeptidyl isomerase A, and a set of stably expressed genes as reference mRNAs in urate crystal inflammation

Cristina Della Beffa, Frank Klawonn, Joseph P Menetski, H Ralph Schumacher, Frank Pessler BMC Research Notes 2011, 4:443 (25 October 2011)

Research article   Open Access

Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues

Muhammad Uddin, Mehmet Cinar, Dawit Tesfaye, Christian Looft, Ernst Tholen, Karl Schellander BMC Research Notes 2011, 4:441 (24 October 2011)

Research article   Open Access

Selection of reference genes for qPCR in hairy root cultures of peanut

Jose Condori, Cesar Nopo-Olazabal, Giuliana Medrano, Fabricio Medina-Bolivar BMC Research Notes 2011, 4:392 (10 October 2011)

Technical Note   Open Access

Reference genes for gene expression studies in wheat flag leaves grown under different farming conditions

Gabriela N Tenea, Adrian Peres Bota, Fernando Cordeiro Raposo, Alain Maquet BMC Research Notes 2011, 4:373 (27 September 2011)

Research article   Open Access

Differential transcriptomic profiles effected by oil palm phenolics indicate novel health outcomes

Soon-Sen Leow, Shamala Sekaran, Kalyana Sundram, YewAi Tan, Ravigadevi Sambanthamurthi BMC Genomics 2011, 12:432 (25 August 2011)

Methodology article   Open Access

Efficiency clustering for low-density microarrays and its application to QPCR

Eric F Lock, Ryan Ziemiecki, JS Marron, Dirk P Dittmer BMC Bioinformatics 2010, 11:386 (20 July 2010)

Short Report   Open Access

An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV

Andrea A Peña, Niels C Bols, Sergio H Marshall BMC Research Notes 2010, 3:101 (14 April 2010)

This article is part of a collection on Quantitative real time PCR...

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Globin-like proteins in Caenorhabditis elegans: in vivo localization, ligand binding and structural properties

Eva Geuens, David Hoogewijs, Marco Nardini, Evi Vinck, Alessandra Pesce, Laurent Kiger, Angela Fago, Lesley Tilleman, Sasha De Henau, Michael C Marden, Roy E Weber, Sabine Van Doorslaer, Jacques Vanfleteren, Luc Moens, Martino Bolognesi, Sylvia Dewilde BMC Biochemistry 2010, 11:17 (2 April 2010)

A detailed physiochemical analysis of two globin-like proteins in Caenorhabditis elegans, GLB-1 and GLB-26, together with the crystal structure of GLB-1*, reveals that these two proteins belong to functionally different globin classes.

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Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data

Sinara Artico, Sarah M Nardeli, Osmundo Brilhante, Maria Grossi-de-Sa, Marcio Alves-Ferreira BMC Plant Biology 2010, 10:49 (21 March 2010)

Research article   Open Access

The embryonic genes Dkk3, Hoxd8, Hoxd9 and Tbx1 identify muscle types in a diet-independent and fiber-type unrelated way

Janneke de Wilde, Martijn FM Hulshof, Mark V Boekschoten, Philip de Groot, Egbert Smit, Edwin CM Mariman BMC Genomics 2010, 11:176 (15 March 2010)

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Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis

Ian Sudbery, Anton J Enright, Andrew G Fraser, Ian Dunham BMC Genomics 2010, 11:175 (15 March 2010)

Research   Open Access

Genome-wide analysis of the diatom cell cycle unveils a novel type of cyclins involved in environmental signaling

Marie JJ Huysman, Cindy Martens, Klaas Vandepoele, Jeroen Gillard, Edda Rayko, Marc Heijde, Chris Bowler, Dirk Inzé, Yves Peer, Lieven De Veylder, Wim Vyverman Genome Biology 2010, 11:R17 (8 February 2010)

Genes controlling the cell cycle in two diatoms have been identified and functionally characterized, revealing environmental regulation of the cell cycle.

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Identification of endogenous control genes for normalisation of real-time quantitative PCR data in colorectal cancer

Elrasheid AH Kheirelseid, Kah Chang, John Newell, Michael J Kerin, Nicola Miller BMC Molecular Biology 2010, 11:12 (1 February 2010)

Research article   Open Access

Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida

Izaskun Mallona, Sandra Lischewski, Julia Weiss, Bettina Hause, Marcos Egea-Cortines BMC Plant Biology 2010, 10:4 (7 January 2010)

Research article   Open Access

ZnT3 mRNA levels are reduced in Alzheimer's disease post-mortem brain

Nancy Beyer, David TR Coulson, Shirley Heggarty, Rivka Ravid, G Brent Irvine, Jan Hellemans, Janet A Johnston Molecular Neurodegeneration 2009, 4:53 (23 December 2009)

Research article   Open Access

Comprehensive characterization of the DNA amplification at 13q34 in human breast cancer reveals TFDP1 and CUL4A as likely candidate target genes

Lorenzo Melchor, Laura Paula Saucedo-Cuevas, Iván Muñoz-Repeto, Socorro María Rodríguez-Pinilla, Emiliano Honrado, Alfredo Campoverde, Jose Palacios, Katherine L Nathanson, María José García, Javier Benítez Breast Cancer Research 2009, 11:R86 (8 December 2009)

Research article   Open Access

Target gene approaches: Gene expression in Daphnia magna exposed to predator-borne kairomones or to microcystin-producing and microcystin-free Microcystis aeruginosa

Anke Schwarzenberger, Cornelius Courts, Eric von Elert BMC Genomics 2009, 10:527 (16 November 2009)

This article is part of a collection on Daphnia: the companion...

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Clinical relevance of nine transcriptional molecular markers for the diagnosis of head and neck squamous cell carcinoma in tissue and saliva rinse

Benjamin Lallemant, Alexandre Evrard, Christophe Combescure, Heliette Chapuis, Guillaume Chambon, Caroline Raynal, Christophe Reynaud, Omar Sabra, Dominique Joubert, Frédéric Hollande, Jean-Gabriel Lallemant, Serge Lumbroso, Jean-Paul Brouillet BMC Cancer 2009, 9:370 (18 October 2009)

Software   Open Access Highly Accessed

QPCR: Application for real-time PCR data management and analysis

Stephan Pabinger, Gerhard G Thallinger, René Snajder, Heiko Eichhorn, Robert Rader, Zlatko Trajanoski BMC Bioinformatics 2009, 10:268 (27 August 2009)

Research article   Open Access

Reference gene selection for head and neck squamous cell carcinoma gene expression studies

Benjamin Lallemant, Alexandre Evrard, Christophe Combescure, Heliette Chapuis, Guillaume Chambon, Caroline Raynal, Christophe Reynaud, Omar Sabra, Dominique Joubert, Frédéric Hollande, Jean-Gabriel Lallemant, Serge Lumbroso, Jean-Paul Brouillet BMC Molecular Biology 2009, 10:78 (3 August 2009)

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Selection of reliable reference genes for gene expression studies in peach using real-time PCR

Zhaoguo Tong, Zhihong Gao, Fei Wang, Jun Zhou, Zhen Zhang BMC Molecular Biology 2009, 10:71 (20 July 2009)

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Selection and evaluation of reference genes for improved interrogation of microbial transcriptomes: case study with the extremophile Acidithiobacillus ferrooxidans

Pamela A Nieto, Paulo C Covarrubias, Eugenia Jedlicki, David S Holmes, Raquel Quatrini BMC Molecular Biology 2009, 10:63 (25 June 2009)

Method   Open Access Highly Accessed

A novel and universal method for microRNA RT-qPCR data normalization

Pieter Mestdagh, Pieter Van Vlierberghe, An De Weer, Daniel Muth, Frank Westermann, Frank Speleman, Jo Vandesompele Genome Biology 2009, 10:R64 (16 June 2009)

The mean expression value: a new method for accurate and reliable normalization of microRNA expression data from RT-qPCR experiments.

Research article   Open Access

Validation of commonly used reference genes for sleep-related gene expression studies

Kil S Lee, Tathiana A Alvarenga, Camila Guindalini, Monica L Andersen, Rosa MRPS Castro, Sergio Tufik BMC Molecular Biology 2009, 10:45 (15 May 2009)

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Transcriptional signatures of BALB/c mouse macrophages housing multiplying Leishmania amazonensis amastigotes

José Fortéa, Emilie de La Llave, Béatrice Regnault, Jean-Yves Coppée, Geneviève Milon, Thierry Lang, Eric Prina BMC Genomics 2009, 10:119 (20 March 2009)

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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process

Marino Expósito-Rodríguez, Andrés A Borges, Andrés Borges-Pérez, José A Pérez BMC Plant Biology 2008, 8:131 (22 December 2008)

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Multiplex ligation-dependent probe amplification for genetic screening in autism spectrum disorders: Efficient identification of known microduplications and identification of a novel microduplication in ASMT

Guiqing Cai, Lisa Edelmann, Juliet E Goldsmith, Ninette Cohen, Alisa Nakamine, Jennifer G Reichert, Ellen J Hoffman, Danielle M Zurawiecki, Jeremy M Silverman, Eric Hollander, Latha Soorya, Evdokia Anagnostou, Catalina Betancur, Joseph D Buxbaum BMC Medical Genomics 2008, 1:50 (16 October 2008)

Multiplex ligation-dependent probe amplification (MPLA) identifies known and novel chromosomal abnormalities in children with autism spectrum disorders and associated cognitive impairment, suggesting that MPLA has the potential to be an effective screening method for these conditions.

Research   Open Access

Pioglitazone administration alters ovarian gene expression in aging obese lethal yellow mice

John D Brannian, Kathleen M Eyster, Mitch Weber, Maureen Diggins Reproductive Biology and Endocrinology 2008, 6:10 (18 March 2008)

Research article   Open Access

Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans

David Hoogewijs, Koen Houthoofd, Filip Matthijssens, Jo Vandesompele, Jacques R Vanfleteren BMC Molecular Biology 2008, 9:9 (22 January 2008)

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Identification of genes for normalization of real-time RT-PCR data in breast carcinomas

Maria B Lyng, Anne-Vibeke Lænkholm, Niels Pallisgaard, Henrik J Ditzel BMC Cancer 2008, 8:20 (22 January 2008)