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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

Jan Hellemans, Geert Mortier, Anne De Paepe, Frank Speleman and Jo Vandesompele*

Author Affiliations

Center for Medical Genetics, Ghent University Hospital, De Pintelaan, B-9000 Ghent, Belgium

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Genome Biology 2007, 8:R19  doi:10.1186/gb-2007-8-2-r19

Published: 9 February 2007

Abstract

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.