Experimental data comparing sample and gene maximization. The sample maximization approach (run 5) is compared to the gene maximization approach (runs 1 and 2 or 1 and 3). The difference between the IRCs is 0.77 for the Cq values, 72% for the NRQ values, and eliminated after inter-run calibration. Grey and white within the same display item indicates that data comes from different runs.
Hellemans et al. Genome Biology 2007 8:R19 doi:10.1186/gb-2007-8-2-r19