Figure 2.

Comparison of genomic prediction with proteomic results (example of ICDS0040). (a) Representation of the DNA region and its predicted ORFs (in color). (b) Detailed view of the two-dimensional gel. Nano-LC-MS-MS data are obtained after extraction and digestion of the protein. The matching peptides are boxed in the translated genomic sequence (a,c). (c) Representation of the DNA region and its predicted ORF upon correction of the sequencing errors (depicted in the ellipse). Correction of the sequencing errors reassociates the two peptides to give a single protein, accounting for their appearance at a single spot.