The genome of Apis mellifera: dialog between linkage mapping and sequence assembly

Michel Solignac¹ , Lan Zhang², Florence Mougel¹, Bingshan Li², Dominique Vautrin¹, Monique Monnerot¹, Jean-Marie Cornuet³, Kim C Worley², George M Weinstock² and Richard A Gibbs²

¹Laboratoire Evolution, Génomes et Spéciation, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette cedex, France and University of Paris Sud, 91405 Orsay, France

²Human Genome Sequencing Center, Baylor College of Medicine, Alkek 1519, One Baylor Plaza, Houston, TX 77030, USA

³Centre de Biologie et de Gestion des Populations, INRA, CS 30016 Montferrier-sur-Lez, 34988 Saint-Gélydu-Fesc, France

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Genome Biology 2007, 8:403doi:10.1186/gb-2007-8-3-403


Published:	19 March 2007

Subject areas: Genome studies

Abstract

Two independent genome projects for the honey bee, a microsatellite linkage map and a genome sequence assembly, interactively produced an almost complete organization of the euchromatic genome. Assembly 4.0 now includes 626 scaffolds that were ordered and oriented into chromosomes according to the framework provided by the third-generation linkage map (AmelMap3). Each construct was used to control the quality of the other. The co-linearity of markers in the sequence and the map is almost perfect and argues in favor of the high quality of both.