Additional data file 3.

(a) Schematic showing the 0.51 Mb and 1.2 Mb homologues and their respective truncated products C1 (0.2 Mb) and C2 (0.9 Mb), with the locations of probes Tc2-Tc5 (Additional data file 5). (b) Autoradiograph illustrating the deletion of the right arms of each chromosome homologue following integration of the fragmentation vector at ORF Tc4. Two clones were isolated after transfection and chromosomal DNA from wild-type (WT) and both clones (C1 and C2) were separated by CHEFE and analyzed by Southern blotting using radiolabeled probe Tc4. Hybridization identifies the larger homologue (1.2 Mb), the smaller homologue (0.51 Mb) and their respective truncated products (0.9 Mb and 0.2 Mb). We had previously shown that probe Tc5 is located a similar distance (approximately 50 kb) from the end of both homologues [22]

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Obado et al. Genome Biology 2007 8:R37   doi:10.1186/gb-2007-8-3-r37