Additional data file 4.
T. brucei procyclic cultures were treated with 500 μM etoposide and chromosomal DNA isolated and fractionated using a Bio-Rad CHEF Mapper system. Membranes were hybridized with probes Tb1-Tb17, the GeneDB systemic names of which are shown in Additional data file 5. The positions of probes (arrows) and locations of the putative centromeric regions (yellow ovals) are indicated. Red arrows indicate INGI/DIRE retroelements and green arrows identify putative ORFs and the implied direction of transcription. The locations of the AT-rich repeat arrays are highlighted (striped box) and the %GC contents across the regions are shown. GeneDB systemic names of ORFs adjacent to the arrays are given and allow the repeat arrays to be mapped onto the chromosomal contigs. Lane N, non-treated parasites; lane E, etoposide-treated. Chromosome sizes were calculated using S. cerevisiae (0.2-2.2 Mb) and Hansenula wingei (1.0-3.1 Mb) chromosome markers (Bio-Rad). In most cases, sequence contigs do not extend to the ends of chromosomes. Gaps in the sequence of AT-rich arrays in chromosomes 3, 6 and 8 are indicated by asterixes and double slashes. We have also found, using long-range restriction mapping (Figures 4b and 5bB), that the 'centromeric-domains' of chromosomes 1 and 4 are larger than predicted by the genome sequence
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Obado et al. Genome Biology 2007 8:R37 doi:10.1186/gb-2007-8-3-r37