Assessing gene expression in (a) liver and (b) WAT. A subset of genes under the transcriptional regulation of Srebp-1c were examined by real-time RT-PCR and, in WAT, were more significantly and strongly up-regulated in the absence of Plscr3. As the goal was to address genotype differences and not individual animal variation, total RNA from three animals/genotype was pooled and technical triplicates were run. *p < 0.01, **p < 0.001.
Mutch et al. Genome Biology 2007 8:R38 doi:10.1186/gb-2007-8-3-r38