Redundancy is dramatically reduced in the reference sequence. Colored bars represent the percentage of Ciona savignyi expressed sequence tags (ESTs) aligning to each assembly a total of zero times (gray bar), exactly once (blue), and exactly twice (yellow). WGS, whole-genome shotgun.
Small et al. Genome Biology 2007 8:R41 doi:10.1186/gb-2007-8-3-r41