Method
CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts
- † Equal contributors
1 Laboratory for Genome Exploration Research Group, Genomic Sciences Center, RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
2 Bioinformatics Solutions Division, Nittetsu Hitachi Systems Engineering, Inc., Akashi-cho, Chuo-ku, Tokyo 104-6591, Japan
3 Genome Science Laboratory, Discovery and Research Institute, RIKEN Wako Main Campus, Hirosawa, Wako 351-0198, Japan
Genome Biology 2007, 8:R42 doi:10.1186/gb-2007-8-3-r42
Published: 26 March 2007Additional files
Additional data file 1:
TSSchip probe annotation and experimental results. Some probe-sequences from the Agilent Catalog Array are not included in this data file because of a material transfer agreement between RIKEN and Agilent. (Please contact Agilent if you need these probe sequences.)
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Additional data file 2:
Shown is the performance of 5'-leading label in dye swap experiments.
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Additional data file 3:
Shown are the details of a sensitivity check with the qRT-PCR.
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Additional data file 4:
Summarized is the sensitivity check with the qRT-PCR.
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Additional data file 5:
Details of the alternative promoter check with qRT-PCR are given.
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Additional data file 6:
Shown are CAGE expression clustering results of Bdh alternative promoters.
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Additional data file 7:
Shown are over-expressed promoters in Hepa1-6 and liver
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Additional data file 8:
Supplementary Methods regarding the array probe design and whole protocols of wet experiments are given.
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Additional data file 9:
Shown are details of cross-validation by qRT-PCR and CAGE in mouse liver versus E17.5.
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