|
Notable experimental studies in the determination of the secretome |
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| Year |
Approach |
Organelle |
Number of proteins |
Comment |
Reference |
|
|
|||||
| 1980 |
Mutagenesis |
Cell-wide |
23 |
First attempt to systematically identify secretory machinery (S. cerevisiae) |
[27] |
| 2000 |
Proteomics |
Golgi |
93 (588)* |
First concerted effort to analyze the Golgi proteome. Cycloheximide treatment used to enrich for Golgi residents |
[7] |
| 2003 |
GFP-tagging |
ER |
296 |
Subcellular localization of more than 75% of the yeast proteome was determined using GFP fusions (S. cerevisiae) |
[3] |
| Golgi/TGN |
118 |
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| 2004 |
Proteomics |
Golgi |
151 (421)† |
Improved proteomics technologies allowed more comprehensive identification of proteins |
[9] |
| 2006 |
Functional assay |
Cell-wide |
130 |
First genome-wide RNAi screen to search for proteins with a functional involvement in secretion (Drosophila S2 cells) |
[26] |
| 2006 |
Proteomics |
ER |
229 |
Proteomics and protein correlation profiling of multiple organelles separated by gradient centrifugation |
[14] |
| ER-Golgi vesicles |
220 |
||||
| Golgi |
67 |
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| 2006 |
GFP-tagging |
ER |
382 (68)‡ |
Subcellular localization of 90% of the yeast proteome was determined using GFP fusions (S. pombe) |
[4] |
| Golgi |
156 (68)‡ |
||||
| 2006 |
Proteomics |
ER |
832 (405)§ |
Most quantitative proteomic characterization of the ER, Golgi and COPI vesicles reported to date |
[13] |
| Golgi |
193 (405)§ |
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|
*Mass spectrometry was used to identify only 93 of the 588 unique spots observed on two-dimensional gels. †From 421 identified proteins, only 151 were annotated as being either unknown or bona fide Golgi residents. ‡An additional 68 proteins localized to the ER and Golgi. §An additional 405 proteins were identified in both ER and Golgi fractions. | |||||
Simpson et al. Genome Biology 2007 8:211 doi:10.1186/gb-2007-8-4-211 |
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