Table 3 |
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|
Primers and PCR conditions |
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|
PigEST name |
Gene symbol |
Oligo sequence (5' to 3') |
Amplicon length |
Tm (°C) |
|
|
||||
|
Ss1.1-rhlv24b_a21.5 |
Troponin |
CCAGAGTCCCCAGGATA TGAGCAGGGCTTTATTTGCATTC |
100 |
63 |
|
Ss1.1-rcst01_n6.5 |
Pepsinogen A precursor |
TACTGCTGCTCAGCTTG GTGTCCTTCAGCTTGCCATTCTT |
106 |
60 |
|
Ss1.1-rcst21_l12.5 |
Pepsinogen C |
TCCTGGTCCTTTTTGACACCTAGAGGACTTGCTGGGGTTG |
108 |
60 |
|
Ss1.1-rhyp08c_e13.5.5 |
Myelin basic protein |
GCAGGGCATAGAGATGGTGTCCCGACCCTGTTAGGAAGAT |
100 |
60 |
|
Ss1.1-Liv1-LVRM1E040203.5.5 |
Fetuin B |
GCCCTGTGTTTCAAATCCTGAGGAGCCACAAGGACAGCTA |
100 |
60 |
|
Ss1.1-rnlu1830b_g11.5 |
SP-C |
TGTACATCTAGGAAACATCAGATTCTTTGGTGGTAGAAGCC |
201 |
60 |
|
Ss1.1-rill310b_f20.5 |
Gastrotropin |
TGAACAGCCCCAACTACCACTCATGCCAGCTTCTTGCTTA |
110 |
60 |
|
Ss1.1-rduo424b_g21.5 |
Vitamin D-dependent calcium binding protein |
TGAGTGCCCAAAAGTCTCCTCAGTTGCTTCAGCTCCTCCT |
153 |
60 |
|
|
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|
Table of the used primers and their corresponding polymerase chain reaction (PCR) conditions for the eight selected genes for which quantitative PCR (qPCR) was carried out. The column 'PigEST name' indicates the name of contig. 'Gene name' is the gene name of the selected genes according to UniProt match. 'Oligo sequence (5' to 3')' is the oligonucleotide sequences used in the qPCR experiment. 'Amplicon length' is the length of the amplified product in the qPCR. 'Tm' indicates the annealing temperature used in the qPCR. |
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|
Gorodkin et al. Genome Biology 2007 8:R45 doi:10.1186/gb-2007-8-4-r45 |
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