Figure 6.

Reversibility of mCat-1 mutations with Cre. (a) Genomic polymerase chain reaction (PCR) with primers for LacZ and Neo detected the βgeo cassette in the five mCat-1 mutations but was absent in five revertants. β-Actin (Actb) served as a positive control for genomic PCR. (b) Five revertant clones were sensitive to G418, confirming excision of the virus and susceptibility to retroviral infection.