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Rossi L
Salvetti A
Marincola FM
Lena A
Deri P
Mannini L
Batistoni R
Wang E
Gremigni V

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Rossi L
Salvetti A
Marincola FM
Lena A
Deri P
Mannini L
Batistoni R
Wang E
Gremigni V
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Research

Deciphering the molecular machinery of stem cells: a look at the neoblast gene expression profile

Leonardo Rossi¹ , Alessandra Salvetti¹ , Francesco M Marincola² , Annalisa Lena¹ , Paolo Deri³ , Linda Mannini³ , Renata Batistoni³ , Ena Wang² and Vittorio Gremigni¹

¹Dipartimento di Morfologia Umana e Biologia Applicata, Sezione di Biologia e Genetica, Università di Pisa, Via Volta, Pisa 56126, Italy

²Department of Transfusion Medicine, Warren G Magnuson Clinical Center, National Institutes of Health, Central Drive, Bethesda, Maryland 20892, USA

³Dipartimento di Biologia, Unità di Biologia Cellulare e dello Sviluppo, Università di Pisa, Via Carducci, Pisa 56010, Italy

author email corresponding author email

Genome Biology 2007, 8:R62doi:10.1186/gb-2007-8-4-r62


Published:	20 April 2007

Subject areas: Development, Molecular biology, Genome studies

Additional files

Additional data file 1:

Shown is a table listing the primers and probes used in the amplification reactions for probe production and real-time RT-PCR analysis.

Format: XLS Size: 21KB Download file

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Additional data file 2:

Shown is a table listing the oligonucleotides included in Dj600 chip.

Format: XLS Size: 153KB Download file

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Additional data file 3:

Shown is a table listing the genes identified with supervised analysis I.

Format: XLS Size: 86KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 4:

The image shows the analysis of the expression of DjMCM2, DjPiwi-1, and DjPum transcripts by real-time RT-PCR after 5 Gy X-ray treatment. RNA was extracted from untreated planarians and the animals were killed 3 or 7 days after 5 Gy X-ray treatment. Expression levels are indicated in relative folds, assuming a value of 1 for untreated specimens. Values are expressed as mean ± standard deviation of three independent samples, conducted in duplicate.

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Additional data file 5:

Shown is a table listing the genes identified with supervised analysis II.

Format: XLS Size: 49KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional data file 6:

The scheme depicts the involvement of several genes found to be upregulated as a consequence of low-dose X-ray treatment in cell death, survival, or motility pathways. Factors found in the 5 Gy specific gene set are boxed in gray; other significant factors are also indicated. The network was deduced based on a literature analysis and is a synthetic representation that does not comprise all of the involved factors. The name of the 5 Gy specific factor is indicated according to principal homology or to alternative names indicated in Additional data file 5.

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Additional data file 7:

The image shows the expression of two selected genes that are differentially regulated in 5 Gy treated planarians compared with 30 Gy irradiated planarians plus controls, visualized by whole mount in situ hybridization in untreated intact planarians (ventral view) and in planarians 4 days after 5 Gy X-ray treatment (ventral view). Scale bar: 500 mm.

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